SUPERFAMILY 1.75 HMM library and genome assignments server


14-3-3 protein superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   All alpha proteins [ 46456] (284)
Fold:   alpha-alpha superhelix [ 48370] (24)
Superfamily:   14-3-3 protein [ 48445]
Families:   14-3-3 protein [ 48446] (4)


Superfamily statistics
Genomes (496) Uniprot 2014_06 PDB chains (SCOP 1.75)
Domains 2,831 3,511 8
Proteins 2,776 3,479 8


Functional annotation
General category coiled coil
Detailed category This is a complex coiled arrangement. The details of which will appear on this page shortly (some coiled coil details are being checked before they are included on the site). If you want to see examples of the states please click here here. If you require further details urgently please contact Owen Rackham

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-quality)

(show details)
GO termFDR (singleton)FDR (all)SDFO levelAnnotation (direct or inherited)
Biological Process (BP)developmental process8.505e-090.0001206Least InformativeDirect
Biological Process (BP)single-organism cellular process1.15e-071.675e-05Least InformativeDirect
Biological Process (BP)localization7.902e-082.759e-07Least InformativeDirect
Biological Process (BP)response to stimulus0.053230.03134Least InformativeInherited
Biological Process (BP)multicellular organismal process3.949e-090.003956Least InformativeInherited
Biological Process (BP)regulation of metabolic process0.32640.7356Least InformativeInherited
Biological Process (BP)regulation of cellular process8.748e-140.03968Least InformativeInherited
Biological Process (BP)negative regulation of metabolic process8.35e-055.361e-05Moderately InformativeDirect
Biological Process (BP)cell differentiation3.89e-102.187e-05Moderately InformativeDirect
Biological Process (BP)macromolecule localization1.39e-107.048e-09Moderately InformativeDirect
Biological Process (BP)regulation of protein metabolic process0.00079792.163e-05Moderately InformativeDirect
Biological Process (BP)cellular localization3.288e-078.367e-09Moderately InformativeDirect
Biological Process (BP)organic substance transport2.729e-054.089e-06Moderately InformativeDirect
Biological Process (BP)regulation of localization0.19540.1725Moderately InformativeInherited
Biological Process (BP)positive regulation of cellular process0.80691Moderately InformativeInherited
Biological Process (BP)regulation of signal transduction0.2420.4421Moderately InformativeInherited
Biological Process (BP)regulation of cellular component organization0.091120.06805Moderately InformativeInherited
Biological Process (BP)regulation of programmed cell death11Moderately InformativeInherited
Biological Process (BP)signal transduction0.71610.3866Moderately InformativeInherited
Biological Process (BP)regulation of developmental process0.053440.4963Moderately InformativeInherited
Biological Process (BP)regulation of multicellular organismal process0.19240.7879Moderately InformativeInherited
Biological Process (BP)regulation of catalytic activity0.27890.005568Moderately InformativeInherited
Biological Process (BP)regulation of phosphate metabolic process12.778e-06Moderately InformativeInherited
Biological Process (BP)negative regulation of cellular process0.014820.01031Moderately InformativeInherited
Biological Process (BP)organ development0.24380.712Moderately InformativeInherited
Biological Process (BP)nervous system development0.0012630.008548Moderately InformativeInherited
Biological Process (BP)protein transport06.378e-06InformativeDirect
Biological Process (BP)regulation of intracellular transport0.00031110.0002137InformativeDirect
Biological Process (BP)regulation of kinase activity3.069e-072.175e-05InformativeDirect
Biological Process (BP)generation of neurons4.7e-111.414e-05InformativeDirect
Biological Process (BP)negative regulation of protein metabolic process0.0009221.427e-06InformativeDirect
Biological Process (BP)regulation of apoptotic signaling pathway4.618e-060.000181InformativeDirect
Biological Process (BP)positive regulation of cellular component organization0.00057630.001772InformativeInherited
Biological Process (BP)regulation of organelle organization0.036260.01264InformativeInherited
Biological Process (BP)regulation of establishment of protein localization1.632e-070.3203InformativeInherited
Biological Process (BP)intracellular signal transduction0.049920.002952InformativeInherited
Biological Process (BP)apoptotic process4.139e-050.001295InformativeInherited
Biological Process (BP)regulation of protein phosphorylation11InformativeInherited
Biological Process (BP)regulation of nervous system development0.035740.1093InformativeInherited
Biological Process (BP)regulation of cell development0.25110.3801InformativeInherited
Biological Process (BP)central nervous system development0.00056040.004527InformativeInherited
Biological Process (BP)cellular component movement0.011650.1221InformativeInherited
Biological Process (BP)locomotion1.154e-050.006285InformativeInherited
Biological Process (BP)localization of cell0.010360.1663InformativeInherited
Biological Process (BP)positive regulation of organelle organization1.94e-050.0001413Highly InformativeDirect
Biological Process (BP)regulation of mitochondrion organization1.103e-091.432e-05Highly InformativeDirect
Biological Process (BP)telencephalon development3.052e-050.0001332Highly InformativeDirect
Biological Process (BP)negative regulation of protein modification process6.708e-051.205e-06Highly InformativeDirect
Biological Process (BP)regulation of cell division7.805e-052.477e-05Highly InformativeDirect
Biological Process (BP)intrinsic apoptotic signaling pathway2.589e-080.0004445Highly InformativeDirect
Molecular Function (MF)binding01.59e-11Least InformativeDirect
Molecular Function (MF)protein domain specific binding1.691e-144.111e-12Highly InformativeDirect
Cellular Component (CC)intracellular organelle part0.37240.7615Least InformativeInherited
Cellular Component (CC)membrane0.053490.204Least InformativeInherited
Cellular Component (CC)cytoplasmic part0.01920.0006Least InformativeInherited
Cellular Component (CC)intracellular membrane-bounded organelle3.164e-090.009823Least InformativeInherited
Cellular Component (CC)vesicle0.0053260.007231Moderately InformativeInherited
Cellular Component (CC)organelle membrane0.002730.005433Moderately InformativeInherited
Cellular Component (CC)cytoplasmic vesicle membrane2.541e-050.000425Highly InformativeDirect

Document: GO annotation of SCOP domains

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) developmental process 0.0001206 Least Informative Direct
Biological Process (BP) single-organism cellular process 1.675e-05 Least Informative Direct
Biological Process (BP) localization 2.759e-07 Least Informative Direct
Biological Process (BP) response to stimulus 0.03134 Least Informative Inherited
Biological Process (BP) multicellular organismal process 0.003956 Least Informative Inherited
Biological Process (BP) regulation of metabolic process 0.7356 Least Informative Inherited
Biological Process (BP) regulation of cellular process 0.03968 Least Informative Inherited
Biological Process (BP) negative regulation of metabolic process 5.361e-05 Moderately Informative Direct
Biological Process (BP) regulation of phosphate metabolic process 2.778e-06 Moderately Informative Direct
Biological Process (BP) cell differentiation 2.187e-05 Moderately Informative Direct
Biological Process (BP) macromolecule localization 7.048e-09 Moderately Informative Direct
Biological Process (BP) regulation of protein metabolic process 2.163e-05 Moderately Informative Direct
Biological Process (BP) cellular localization 8.367e-09 Moderately Informative Direct
Biological Process (BP) organic substance transport 4.089e-06 Moderately Informative Direct
Biological Process (BP) regulation of localization 0.1725 Moderately Informative Inherited
Biological Process (BP) positive regulation of cellular process 1 Moderately Informative Inherited
Biological Process (BP) regulation of signal transduction 0.4421 Moderately Informative Inherited
Biological Process (BP) regulation of cellular component organization 0.06805 Moderately Informative Inherited
Biological Process (BP) regulation of programmed cell death 1 Moderately Informative Inherited
Biological Process (BP) signal transduction 0.3866 Moderately Informative Inherited
Biological Process (BP) regulation of developmental process 0.4963 Moderately Informative Inherited
Biological Process (BP) regulation of multicellular organismal process 0.7879 Moderately Informative Inherited
Biological Process (BP) regulation of catalytic activity 0.005568 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.01031 Moderately Informative Inherited
Biological Process (BP) organ development 0.712 Moderately Informative Inherited
Biological Process (BP) nervous system development 0.008548 Moderately Informative Inherited
Biological Process (BP) cellular response to stress 0.02567 Moderately Informative Inherited
Biological Process (BP) protein transport 6.378e-06 Informative Direct
Biological Process (BP) regulation of intracellular transport 0.0002137 Informative Direct
Biological Process (BP) regulation of kinase activity 2.175e-05 Informative Direct
Biological Process (BP) generation of neurons 1.414e-05 Informative Direct
Biological Process (BP) negative regulation of protein metabolic process 1.427e-06 Informative Direct
Biological Process (BP) regulation of apoptotic signaling pathway 0.000181 Informative Direct
Biological Process (BP) positive regulation of cellular component organization 0.001772 Informative Inherited
Biological Process (BP) regulation of organelle organization 0.01264 Informative Inherited
Biological Process (BP) regulation of establishment of protein localization 0.3203 Informative Inherited
Biological Process (BP) intracellular signal transduction 0.002952 Informative Inherited
Biological Process (BP) apoptotic process 0.001295 Informative Inherited
Biological Process (BP) regulation of protein phosphorylation 1 Informative Inherited
Biological Process (BP) regulation of nervous system development 0.1093 Informative Inherited
Biological Process (BP) regulation of cell development 0.3801 Informative Inherited
Biological Process (BP) regulation of cell cycle process 0.2994 Informative Inherited
Biological Process (BP) central nervous system development 0.004527 Informative Inherited
Biological Process (BP) cellular component movement 0.1221 Informative Inherited
Biological Process (BP) locomotion 0.006285 Informative Inherited
Biological Process (BP) localization of cell 0.1663 Informative Inherited
Biological Process (BP) response to DNA damage stimulus 0.01884 Informative Inherited
Biological Process (BP) DNA damage checkpoint 1.006e-05 Highly Informative Direct
Biological Process (BP) positive regulation of organelle organization 0.0001413 Highly Informative Direct
Biological Process (BP) regulation of mitochondrion organization 1.432e-05 Highly Informative Direct
Biological Process (BP) telencephalon development 0.0001332 Highly Informative Direct
Biological Process (BP) negative regulation of protein modification process 1.205e-06 Highly Informative Direct
Biological Process (BP) regulation of cell division 2.477e-05 Highly Informative Direct
Biological Process (BP) intrinsic apoptotic signaling pathway 0.0004445 Highly Informative Direct
Molecular Function (MF) binding 1.59e-11 Least Informative Direct
Molecular Function (MF) enzyme regulator activity 0.001061 Moderately Informative Inherited
Molecular Function (MF) enzyme inhibitor activity 0.0006085 Informative Direct
Molecular Function (MF) protein domain specific binding 4.111e-12 Highly Informative Direct
Cellular Component (CC) cytoplasmic part 0.0006 Least Informative Direct
Cellular Component (CC) intracellular organelle part 0.7615 Least Informative Inherited
Cellular Component (CC) membrane 0.204 Least Informative Inherited
Cellular Component (CC) intracellular membrane-bounded organelle 0.009823 Least Informative Inherited
Cellular Component (CC) vesicle 0.007231 Moderately Informative Inherited
Cellular Component (CC) organelle membrane 0.005433 Moderately Informative Inherited
Cellular Component (CC) cytoplasmic vesicle membrane 0.000425 Highly Informative Direct

Document: GO annotation of SCOP domains

Disease Ontology (DO)

(show details)
DO termFDR (all)SDDO levelAnnotation (direct or inherited)
Disease Ontology (DO)organ system cancer0Least InformativeDirect
Disease Ontology (DO)nervous system cancer0Moderately InformativeDirect
Disease Ontology (DO)disease of mental health0.003241Moderately InformativeInherited
Disease Ontology (DO)meningioma0InformativeDirect
Disease Ontology (DO)schizophrenia5.728e-05InformativeDirect

Document: DO annotation of SCOP domains

Xenopus Anatomy (XA)

(show details)
XA termFDR (all)SDXA levelAnnotation (direct or inherited)
Xenopus ANatomical entity (XAN)embryo0Least InformativeDirect
Xenopus ANatomical entity (XAN)genital system0Least InformativeDirect
Xenopus ANatomical entity (XAN)central nervous system0Least InformativeDirect
Xenopus ANatomical entity (XAN)head0Least InformativeDirect
Xenopus ANatomical entity (XAN)viscus0Least InformativeDirect
Xenopus ANatomical entity (XAN)portion of tissue0Least InformativeDirect
Xenopus ANatomical entity (XAN)ectoderm0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)surface structure0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)embryonic structure0.003978Moderately InformativeInherited
Xenopus ANatomical entity (XAN)skin0InformativeDirect
Xenopus ANatomical entity (XAN)marginal zone0.0002662Highly InformativeDirect

Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details)
AP termFDR (all)SDAP levelAnnotation (direct or inherited)
Plant structure DEvelopment stage (PDE)seed development stage1.059e-07InformativeDirect

Document: AP annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Cellular componentCytoplasm6.214e-12Least InformativeDirect
Coding sequence diversityAlternative initiation7.745e-06Moderately InformativeDirect
Post-translational modificationAcetylation0Least InformativeDirect
Post-translational modificationPhosphoprotein1.009e-06Least InformativeDirect
Post-translational modificationNitration3.48e-09InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000308 SSF48445 Protein matches
Abstract

The 14-3-3 proteins are a large family of approximately 30kDa acidic proteins which exist primarily as homo- and heterodimeric within all eukaryotic cells [PubMed1671102, PubMed11911880]. There is a high degree of sequence identity and conservation between all the 14-3-3 isotypes, particularly in the regions which form the dimer interface or line the central ligand binding channel of the dimeric molecule. Each 14-3-3 protein sequence can be roughly divided into three sections: a divergent amino terminus, the conserved core region and a divergent carboxyl terminus. The conserved middle core region of the 14-3-3s encodes an amphipathic groove that forms the main functional domain, a cradle for interacting with client proteins. The monomer consists of nine helices organised in an antiparallel manner, forming an L-shaped structure. The interior of the L-structure is composed of four helices: H3 and H5, which contain many charged and polar amino acids, and H7 and H9, which contain hydrophobic amino acids. These four helices form the concave amphipathic groove that interacts with target peptides.

14-3-3 proteins mainly bind proteins containing phosphothreonine or phosphoserine motifs however exceptions to this rule do exist. Extensive investigation of the 14-3-3 binding site of the mammalian serine/threonine kinase Raf-1 has produced a consensus sequence for 14-3-3-binding, RSxpSxP (in the single-letter amino-acid code, where x denotes any amino acid and p indicates that the next residue is phosphorylated). 14-3-3 proteins appear to effect intracellular signalling in one of three ways - by direct regulation of the catalytic activity of the bound protein, by regulating interactions between the bound protein and other molecules in the cell by sequestration or modification or by controlling the subcellular localisation of the bound ligand. Proteins appear to initially bind to a single dominant site and then subsequently to many, much weaker secondary interaction sites. The 14-3-3 dimer is capable of changing the conformation of its bound ligand whilst itself undergoing minimal structural alteration.


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 4 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a 14-3-3 protein domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 4 hidden Markov models representing the 14-3-3 protein superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · Internal database links ]