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14-3-3 protein superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   All alpha proteins [ 46456] (284)
Fold:   alpha-alpha superhelix [ 48370] (24)
Superfamily:   14-3-3 protein [ 48445]
Families:   14-3-3 protein [ 48446] (4)


Superfamily statistics
Genomes (503) Uniprot 2017_06 genome PDB chains (SCOP 1.75)
Domains 2,885 6,867 8
Proteins 2,827 6,755 8


Functional annotation
General category coiled coil
Detailed category This is a complex coiled arrangement. The details of which will appear on this page shortly (some coiled coil details are being checked before they are included on the site). If you want to see examples of the states please click here here. If you require further details urgently please contact Owen Rackham

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-quality)

(show details)
GO termFDR (singleton)FDR (all)SDFO levelAnnotation (direct or inherited)
Biological Process (BP)response to stimulus0.00041840.0008733Least InformativeDirect
Biological Process (BP)biological regulation0.000000000000045140.000001591Least InformativeDirect
Biological Process (BP)cellular component organization or biogenesis0.000008350.01019Least InformativeInherited
Biological Process (BP)single-organism cellular process0.10910.1978Least InformativeInherited
Biological Process (BP)reproduction0.000067850.0003916Moderately InformativeDirect
Biological Process (BP)cell communication0.00000012280.0001669Moderately InformativeDirect
Biological Process (BP)single organism signaling0.000002770.0003359Moderately InformativeDirect
Biological Process (BP)regulation of localization0.36240.2914Moderately InformativeInherited
Biological Process (BP)cellular developmental process0.0000021010.001162Moderately InformativeInherited
Biological Process (BP)system development0.10770.7284Moderately InformativeInherited
Biological Process (BP)cellular response to chemical stimulus0.46930.7744Moderately InformativeInherited
Biological Process (BP)response to oxygen-containing compound0.26720.3569Moderately InformativeInherited
Biological Process (BP)response to organic substance0.026030.5217Moderately InformativeInherited
Biological Process (BP)anatomical structure morphogenesis0.4630.5733Moderately InformativeInherited
Biological Process (BP)response to abiotic stimulus0.28170.4257Moderately InformativeInherited
Biological Process (BP)macromolecule localization0.018110.02085Moderately InformativeInherited
Biological Process (BP)response to temperature stimulus0.000042390.0002881InformativeDirect
Biological Process (BP)response to organic cyclic compound0.00002660.00002554InformativeDirect
Biological Process (BP)cellular response to hormone stimulus0.0000011130.00001194InformativeDirect
Biological Process (BP)growth0.00014440.0000343InformativeDirect
Biological Process (BP)single organism reproductive process0.000085530.0004726InformativeDirect
Biological Process (BP)regulation of establishment of protein localization0.0000048780.00004068InformativeDirect
Biological Process (BP)cellular response to lipid0.00032240.00004412InformativeDirect
Biological Process (BP)cellular response to oxygen-containing compound0.0007160.0006022InformativeDirect
Biological Process (BP)regulation of cellular localization0.0022530.001433InformativeInherited
Biological Process (BP)tissue development0.23310.5562InformativeInherited
Biological Process (BP)post-embryonic development0.0038190.00196InformativeInherited
Biological Process (BP)reproductive system development0.0068350.006374InformativeInherited
Biological Process (BP)cell development0.037960.02263InformativeInherited
Biological Process (BP)multicellular organism reproduction0.011950.01307InformativeInherited
Biological Process (BP)developmental process involved in reproduction0.011590.002326InformativeInherited
Biological Process (BP)cellular component morphogenesis0.0043990.03494InformativeInherited
Biological Process (BP)hormone-mediated signaling pathway0.000062120.00003027Highly InformativeDirect
Biological Process (BP)trichome differentiation0.000013060.0000000966Highly InformativeDirect
Biological Process (BP)cell growth0.00000032350.00000007026Highly InformativeDirect
Biological Process (BP)seed development0.000011240.00000362Highly InformativeDirect
Biological Process (BP)regulation of cell division0.00014040.00002012Highly InformativeDirect
Biological Process (BP)developmental growth involved in morphogenesis0.0000022730.00002173Highly InformativeDirect
Biological Process (BP)cellular response to steroid hormone stimulus0.000015830.000001527Highly InformativeDirect
Biological Process (BP)positive regulation of cellular protein localization0.0000072590.00003172Highly InformativeDirect
Biological Process (BP)cell morphogenesis involved in differentiation0.0026020.04246Highly InformativeInherited
Molecular Function (MF)binding0.0000000000011260.0000001364Least InformativeDirect
Molecular Function (MF)molecular function regulator0.000010690.000006848Moderately InformativeDirect
Molecular Function (MF)enzyme binding0.00034230.01308InformativeInherited
Molecular Function (MF)protein dimerization activity0.16640.1178InformativeInherited
Molecular Function (MF)enzyme inhibitor activity0.049830.000469InformativeInherited
Molecular Function (MF)protein kinase regulator activity0.0000000097550.000000008148Highly InformativeDirect
Molecular Function (MF)protein domain specific binding0.00000000018060.0000004366Highly InformativeDirect
Molecular Function (MF)protein heterodimerization activity0.00000000036750.0000001108Highly InformativeDirect
Cellular Component (CC)membrane0.063330.1196Least InformativeInherited
Cellular Component (CC)cytoplasmic part0.24770.01258Least InformativeInherited
Cellular Component (CC)intracellular organelle part0.57720.9705Least InformativeInherited
Cellular Component (CC)vesicle0.020340.07623Moderately InformativeInherited
Cellular Component (CC)cytoplasmic vesicle part0.000065070.001033InformativeInherited
Cellular Component (CC)cytoplasmic vesicle membrane0.000000018680.000004534Highly InformativeDirect

Document: GO annotation of SCOP domains

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) response to stimulus 0.0008733 Least Informative Direct
Biological Process (BP) biological regulation 0.000001591 Least Informative Direct
Biological Process (BP) cellular component organization or biogenesis 0.01019 Least Informative Inherited
Biological Process (BP) single-organism cellular process 0.1978 Least Informative Inherited
Biological Process (BP) reproduction 0.0003916 Moderately Informative Direct
Biological Process (BP) cell communication 0.0001669 Moderately Informative Direct
Biological Process (BP) single organism signaling 0.0003359 Moderately Informative Direct
Biological Process (BP) regulation of localization 0.2914 Moderately Informative Inherited
Biological Process (BP) cellular developmental process 0.001162 Moderately Informative Inherited
Biological Process (BP) system development 0.7284 Moderately Informative Inherited
Biological Process (BP) cellular response to chemical stimulus 0.7744 Moderately Informative Inherited
Biological Process (BP) response to oxygen-containing compound 0.3569 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.3362 Moderately Informative Inherited
Biological Process (BP) response to organic substance 0.5217 Moderately Informative Inherited
Biological Process (BP) anatomical structure morphogenesis 0.5733 Moderately Informative Inherited
Biological Process (BP) response to abiotic stimulus 0.4257 Moderately Informative Inherited
Biological Process (BP) macromolecule localization 0.02085 Moderately Informative Inherited
Biological Process (BP) organelle organization 0.8811 Moderately Informative Inherited
Biological Process (BP) cellular localization 0.01788 Moderately Informative Inherited
Biological Process (BP) cellular response to stress 0.1161 Moderately Informative Inherited
Biological Process (BP) mitochondrion organization 0.0002506 Informative Direct
Biological Process (BP) response to temperature stimulus 0.0002881 Informative Direct
Biological Process (BP) response to organic cyclic compound 0.00002554 Informative Direct
Biological Process (BP) cell cycle process 0.0003886 Informative Direct
Biological Process (BP) cellular response to hormone stimulus 0.00001194 Informative Direct
Biological Process (BP) growth 0.0000343 Informative Direct
Biological Process (BP) single organism reproductive process 0.0004726 Informative Direct
Biological Process (BP) regulation of cell cycle 0.0004204 Informative Direct
Biological Process (BP) regulation of establishment of protein localization 0.00004068 Informative Direct
Biological Process (BP) cellular response to lipid 0.00004412 Informative Direct
Biological Process (BP) cellular response to oxygen-containing compound 0.0006022 Informative Direct
Biological Process (BP) regulation of cellular localization 0.001433 Informative Inherited
Biological Process (BP) tissue development 0.5562 Informative Inherited
Biological Process (BP) post-embryonic development 0.00196 Informative Inherited
Biological Process (BP) reproductive system development 0.006374 Informative Inherited
Biological Process (BP) cell development 0.02263 Informative Inherited
Biological Process (BP) multicellular organism reproduction 0.01307 Informative Inherited
Biological Process (BP) developmental process involved in reproduction 0.002326 Informative Inherited
Biological Process (BP) cellular component morphogenesis 0.03494 Informative Inherited
Biological Process (BP) cellular response to DNA damage stimulus 0.06136 Informative Inherited
Biological Process (BP) cell cycle checkpoint 0.00000004132 Highly Informative Direct
Biological Process (BP) mitochondrial transport 0.0002812 Highly Informative Direct
Biological Process (BP) hormone-mediated signaling pathway 0.00003027 Highly Informative Direct
Biological Process (BP) trichome differentiation 0.0000000966 Highly Informative Direct
Biological Process (BP) cell growth 0.00000007026 Highly Informative Direct
Biological Process (BP) seed development 0.00000362 Highly Informative Direct
Biological Process (BP) regulation of cell division 0.00002012 Highly Informative Direct
Biological Process (BP) developmental growth involved in morphogenesis 0.00002173 Highly Informative Direct
Biological Process (BP) cellular response to steroid hormone stimulus 0.000001527 Highly Informative Direct
Biological Process (BP) negative regulation of cell cycle phase transition 0.000002582 Highly Informative Direct
Biological Process (BP) positive regulation of cellular protein localization 0.00003172 Highly Informative Direct
Biological Process (BP) negative regulation of mitotic cell cycle 0.04916 Highly Informative Inherited
Biological Process (BP) cell morphogenesis involved in differentiation 0.04246 Highly Informative Inherited
Molecular Function (MF) binding 0.0000001364 Least Informative Direct
Molecular Function (MF) molecular function regulator 0.000006848 Moderately Informative Direct
Molecular Function (MF) enzyme inhibitor activity 0.000469 Informative Direct
Molecular Function (MF) enzyme binding 0.01308 Informative Inherited
Molecular Function (MF) protein dimerization activity 0.1178 Informative Inherited
Molecular Function (MF) protein kinase regulator activity 0.000000008148 Highly Informative Direct
Molecular Function (MF) protein domain specific binding 0.0000004366 Highly Informative Direct
Molecular Function (MF) protein heterodimerization activity 0.0000001108 Highly Informative Direct
Cellular Component (CC) membrane 0.1196 Least Informative Inherited
Cellular Component (CC) intracellular organelle part 0.9705 Least Informative Inherited
Cellular Component (CC) cytoplasmic part 0.01258 Least Informative Inherited
Cellular Component (CC) vesicle 0.07623 Moderately Informative Inherited
Cellular Component (CC) external encapsulating structure 0.0007136 Informative Direct
Cellular Component (CC) cytoplasmic vesicle part 0.001033 Informative Inherited
Cellular Component (CC) cytoplasmic vesicle membrane 0.000004534 Highly Informative Direct

Document: GO annotation of SCOP domains

Disease Ontology (DO)

(show details)
DO termFDR (all)SDDO levelAnnotation (direct or inherited)
Disease Ontology (DO)organ system cancer0Least InformativeDirect
Disease Ontology (DO)nervous system cancer0Moderately InformativeDirect
Disease Ontology (DO)disease of mental health0.001678Moderately InformativeInherited
Disease Ontology (DO)meningioma0InformativeDirect
Disease Ontology (DO)schizophrenia0.00005654InformativeDirect

Document: DO annotation of SCOP domains

Worm Phenotype (WP)

(show details)
WP termFDR (all)SDWP levelAnnotation (direct or inherited)
Worm Phenotype (WP)organism metabolism processing variant0Least InformativeDirect
Worm Phenotype (WP)larval lethal0Least InformativeDirect
Worm Phenotype (WP)retarded heterochronic variations0Least InformativeDirect
Worm Phenotype (WP)cell development variant0Least InformativeDirect
Worm Phenotype (WP)organ system morphology variant0Least InformativeDirect
Worm Phenotype (WP)cell physiology variant0Least InformativeDirect
Worm Phenotype (WP)larval development variant0Least InformativeDirect
Worm Phenotype (WP)larval growth variant0Least InformativeDirect
Worm Phenotype (WP)protein expression variant0Moderately InformativeDirect
Worm Phenotype (WP)reproductive system morphology variant0Moderately InformativeDirect
Worm Phenotype (WP)gametogenesis variant0Moderately InformativeDirect
Worm Phenotype (WP)pattern of transgene expression variant0Moderately InformativeDirect
Worm Phenotype (WP)level of transgene expression variant0Moderately InformativeDirect
Worm Phenotype (WP)sterile progeny0Moderately InformativeDirect
Worm Phenotype (WP)protein protein interaction variant0Moderately InformativeDirect
Worm Phenotype (WP)life span variant0InformativeDirect
Worm Phenotype (WP)dauer arrest variant0InformativeDirect
Worm Phenotype (WP)pattern protein expression variant0InformativeDirect
Worm Phenotype (WP)endocytic transport defect0InformativeDirect
Worm Phenotype (WP)oocyte physiology variant0InformativeDirect
Worm Phenotype (WP)enzyme activity reduced0Highly InformativeDirect
Worm Phenotype (WP)shortened life span0Highly InformativeDirect
Worm Phenotype (WP)transgene expression reduced0Highly InformativeDirect

Document: WP annotation of SCOP domains

Xenopus Anatomy (XA)

(show details)
XA termFDR (all)SDXA levelAnnotation (direct or inherited)
Xenopus ANatomical entity (XAN)embryo0Least InformativeDirect
Xenopus ANatomical entity (XAN)genital system0Least InformativeDirect
Xenopus ANatomical entity (XAN)nervous system0Least InformativeDirect
Xenopus ANatomical entity (XAN)head0Least InformativeDirect
Xenopus ANatomical entity (XAN)tissue0Least InformativeDirect
Xenopus ANatomical entity (XAN)anatomical cluster0Least InformativeDirect
Xenopus ANatomical entity (XAN)ectoderm0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)brain0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)multi-tissue structure0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)embryonic structure0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)visual system0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)immune system0.02046Moderately InformativeInherited
Xenopus ANatomical entity (XAN)alimentary system0.1087Moderately InformativeInherited
Xenopus ANatomical entity (XAN)skin0InformativeDirect
Xenopus ANatomical entity (XAN)pharyngeal region0InformativeDirect
Xenopus ANatomical entity (XAN)peripheral nervous system0.00000008301InformativeDirect
Xenopus ANatomical entity (XAN)spinal cord0.000002911InformativeDirect
Xenopus ANatomical entity (XAN)endocrine system0.0006046InformativeDirect
Xenopus ANatomical entity (XAN)mesoderm0.00806InformativeInherited
Xenopus ANatomical entity (XAN)ganglion0.0000008975Highly InformativeDirect
Xenopus ANatomical entity (XAN)somite0.000265Highly InformativeDirect
Xenopus ANatomical entity (XAN)mesenchyme0.0004924Highly InformativeDirect
Xenopus ANatomical entity (XAN)epidermis0.0007513Highly InformativeDirect

Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details)
AP termFDR (all)SDAP levelAnnotation (direct or inherited)
Plant structure DEvelopment stage (PDE)seed development stage0.0000001061InformativeDirect

Document: AP annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Cellular componentCytoplasm0Least InformativeDirect
Cellular componentNucleus0.0000001046Least InformativeDirect
Coding sequence diversityAlternative initiation0.000007825Moderately InformativeDirect
Post-translational modificationAcetylation0Least InformativeDirect
Post-translational modificationPhosphoprotein0.00001164Least InformativeDirect
Post-translational modificationNitration0.000000004689InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000308 SSF48445 Protein matches
Abstract

The 14-3-3 proteins are a large family of approximately 30kDa acidic proteins which exist primarily as homo- and heterodimeric within all eukaryotic cells [PubMed1671102, PubMed11911880]. There is a high degree of sequence identity and conservation between all the 14-3-3 isotypes, particularly in the regions which form the dimer interface or line the central ligand binding channel of the dimeric molecule. Each 14-3-3 protein sequence can be roughly divided into three sections: a divergent amino terminus, the conserved core region and a divergent carboxyl terminus. The conserved middle core region of the 14-3-3s encodes an amphipathic groove that forms the main functional domain, a cradle for interacting with client proteins. The monomer consists of nine helices organised in an antiparallel manner, forming an L-shaped structure. The interior of the L-structure is composed of four helices: H3 and H5, which contain many charged and polar amino acids, and H7 and H9, which contain hydrophobic amino acids. These four helices form the concave amphipathic groove that interacts with target peptides.

14-3-3 proteins mainly bind proteins containing phosphothreonine or phosphoserine motifs however exceptions to this rule do exist. Extensive investigation of the 14-3-3 binding site of the mammalian serine/threonine kinase Raf-1 has produced a consensus sequence for 14-3-3-binding, RSxpSxP (in the single-letter amino-acid code, where x denotes any amino acid and p indicates that the next residue is phosphorylated). 14-3-3 proteins appear to effect intracellular signalling in one of three ways - by direct regulation of the catalytic activity of the bound protein, by regulating interactions between the bound protein and other molecules in the cell by sequestration or modification or by controlling the subcellular localisation of the bound ligand. Proteins appear to initially bind to a single dominant site and then subsequently to many, much weaker secondary interaction sites. The 14-3-3 dimer is capable of changing the conformation of its bound ligand whilst itself undergoing minimal structural alteration.


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Worm Phenotype (WP) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 4 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a 14-3-3 protein domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 4 hidden Markov models representing the 14-3-3 protein superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Worm Phenotype (WP) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · Internal database links ]