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14-3-3 protein superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   All alpha proteins [ 46456] (284)
Fold:   alpha-alpha superhelix [ 48370] (24)
Superfamily:   14-3-3 protein [ 48445]
Families:   14-3-3 protein [ 48446] (4)


Superfamily statistics
Genomes (498) Uniprot 2014_06 PDB chains (SCOP 1.75)
Domains 2,843 3,511 8
Proteins 2,787 3,479 8


Functional annotation
General category coiled coil
Detailed category This is a complex coiled arrangement. The details of which will appear on this page shortly (some coiled coil details are being checked before they are included on the site). If you want to see examples of the states please click here here. If you require further details urgently please contact Owen Rackham

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-quality)

(show details)
GO termFDR (singleton)FDR (all)SDFO levelAnnotation (direct or inherited)
Biological Process (BP)biological regulation0.000000000000038780.000001911Least InformativeDirect
Biological Process (BP)single-organism cellular process0.0023620.07553Least InformativeInherited
Biological Process (BP)cellular component organization or biogenesis0.0000034340.00605Least InformativeInherited
Biological Process (BP)reproduction0.000049310.0002504Moderately InformativeDirect
Biological Process (BP)regulation of localization0.360.3832Moderately InformativeInherited
Biological Process (BP)anatomical structure morphogenesis0.46290.58Moderately InformativeInherited
Biological Process (BP)system development0.11730.7531Moderately InformativeInherited
Biological Process (BP)negative regulation of cellular process0.3770.141Moderately InformativeInherited
Biological Process (BP)cellular developmental process0.000010370.002126Moderately InformativeInherited
Biological Process (BP)regulation of cell communication0.0029610.003337Moderately InformativeInherited
Biological Process (BP)regulation of signaling0.017250.02422Moderately InformativeInherited
Biological Process (BP)regulation of response to stimulus0.53920.4043Moderately InformativeInherited
Biological Process (BP)macromolecule localization0.0091610.01256Moderately InformativeInherited
Biological Process (BP)growth0.00012120.00002504InformativeDirect
Biological Process (BP)single organism reproductive process0.000055250.0002292InformativeDirect
Biological Process (BP)regulation of protein localization0.010470.007551InformativeInherited
Biological Process (BP)regulation of cellular localization0.0017760.003446InformativeInherited
Biological Process (BP)reproductive system development0.0065470.005442InformativeInherited
Biological Process (BP)cell development0.03520.01879InformativeInherited
Biological Process (BP)post-embryonic development0.0038840.002101InformativeInherited
Biological Process (BP)multicellular organism reproduction0.0080110.009085InformativeInherited
Biological Process (BP)negative regulation of cell communication0.024410.2455InformativeInherited
Biological Process (BP)negative regulation of signaling0.028020.2582InformativeInherited
Biological Process (BP)negative regulation of response to stimulus0.04170.3022InformativeInherited
Biological Process (BP)developmental process involved in reproduction0.010530.001976InformativeInherited
Biological Process (BP)cellular component morphogenesis0.0044050.0305InformativeInherited
Biological Process (BP)mitochondrial transport0.000068030.00001071Highly InformativeDirect
Biological Process (BP)regulation of G-protein coupled receptor protein signaling pathway0.00006540.00002208Highly InformativeDirect
Biological Process (BP)trichome differentiation0.000010210.00000008321Highly InformativeDirect
Biological Process (BP)cell growth0.00000070430.00000009455Highly InformativeDirect
Biological Process (BP)seed development0.000010520.000002339Highly InformativeDirect
Biological Process (BP)regulation of cell division0.0001470.00001207Highly InformativeDirect
Biological Process (BP)developmental growth involved in morphogenesis0.0000019970.00001729Highly InformativeDirect
Biological Process (BP)positive regulation of cellular protein localization0.0000078260.0000257Highly InformativeDirect
Biological Process (BP)cell morphogenesis involved in differentiation0.002430.03973Highly InformativeInherited
Molecular Function (MF)binding0.00000000000035740.0000001043Least InformativeDirect
Molecular Function (MF)protein binding00.00001898Moderately InformativeDirect
Molecular Function (MF)molecular function regulator0.0000075650.00000412Moderately InformativeDirect
Molecular Function (MF)enzyme binding0.0012090.02146InformativeInherited
Molecular Function (MF)protein dimerization activity0.13940.07314InformativeInherited
Molecular Function (MF)enzyme inhibitor activity0.048130.0004612InformativeInherited
Molecular Function (MF)protein kinase regulator activity0.0000000055910.000000005511Highly InformativeDirect
Molecular Function (MF)protein heterodimerization activity0.00000000017740.00000006391Highly InformativeDirect
Cellular Component (CC)cytoplasmic part0.27430.01537Least InformativeInherited
Cellular Component (CC)intracellular organelle part0.3670.7126Least InformativeInherited
Cellular Component (CC)membrane0.083020.1544Least InformativeInherited
Cellular Component (CC)organelle membrane0.00020480.000898Moderately InformativeDirect
Cellular Component (CC)vesicle0.010770.03326Moderately InformativeInherited
Cellular Component (CC)cell junction0.00000043670.0001066InformativeDirect
Cellular Component (CC)cytoplasmic vesicle part0.0000044830.0001236InformativeDirect
Cellular Component (CC)vesicle membrane0.0000055170.00006491Highly InformativeDirect

Document: GO annotation of SCOP domains

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) biological regulation 0.000001911 Least Informative Direct
Biological Process (BP) single-organism cellular process 0.07553 Least Informative Inherited
Biological Process (BP) cellular component organization or biogenesis 0.00605 Least Informative Inherited
Biological Process (BP) response to stimulus 0.00603 Least Informative Inherited
Biological Process (BP) reproduction 0.0002504 Moderately Informative Direct
Biological Process (BP) regulation of localization 0.3832 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.141 Moderately Informative Inherited
Biological Process (BP) anatomical structure morphogenesis 0.58 Moderately Informative Inherited
Biological Process (BP) system development 0.7531 Moderately Informative Inherited
Biological Process (BP) cellular developmental process 0.002126 Moderately Informative Inherited
Biological Process (BP) regulation of cell communication 0.003337 Moderately Informative Inherited
Biological Process (BP) regulation of signaling 0.02422 Moderately Informative Inherited
Biological Process (BP) regulation of response to stimulus 0.4043 Moderately Informative Inherited
Biological Process (BP) macromolecule localization 0.01256 Moderately Informative Inherited
Biological Process (BP) response to stress 0.4168 Moderately Informative Inherited
Biological Process (BP) cell cycle process 0.0003967 Informative Direct
Biological Process (BP) growth 0.00002504 Informative Direct
Biological Process (BP) single organism reproductive process 0.0002292 Informative Direct
Biological Process (BP) regulation of cell cycle 0.0001853 Informative Direct
Biological Process (BP) regulation of protein localization 0.007551 Informative Inherited
Biological Process (BP) regulation of cellular localization 0.003446 Informative Inherited
Biological Process (BP) reproductive system development 0.005442 Informative Inherited
Biological Process (BP) cell development 0.01879 Informative Inherited
Biological Process (BP) post-embryonic development 0.002101 Informative Inherited
Biological Process (BP) multicellular organism reproduction 0.009085 Informative Inherited
Biological Process (BP) negative regulation of cell communication 0.2455 Informative Inherited
Biological Process (BP) negative regulation of signaling 0.2582 Informative Inherited
Biological Process (BP) negative regulation of response to stimulus 0.3022 Informative Inherited
Biological Process (BP) developmental process involved in reproduction 0.001976 Informative Inherited
Biological Process (BP) cellular component morphogenesis 0.0305 Informative Inherited
Biological Process (BP) cellular response to DNA damage stimulus 0.04555 Informative Inherited
Biological Process (BP) cell cycle checkpoint 0.00000003057 Highly Informative Direct
Biological Process (BP) mitochondrial transport 0.00001071 Highly Informative Direct
Biological Process (BP) regulation of G-protein coupled receptor protein signaling pathway 0.00002208 Highly Informative Direct
Biological Process (BP) trichome differentiation 0.00000008321 Highly Informative Direct
Biological Process (BP) cell growth 0.00000009455 Highly Informative Direct
Biological Process (BP) seed development 0.000002339 Highly Informative Direct
Biological Process (BP) regulation of cell division 0.00001207 Highly Informative Direct
Biological Process (BP) developmental growth involved in morphogenesis 0.00001729 Highly Informative Direct
Biological Process (BP) positive regulation of cellular protein localization 0.0000257 Highly Informative Direct
Biological Process (BP) negative regulation of mitotic cell cycle 0.05007 Highly Informative Inherited
Biological Process (BP) regulation of mitotic cell cycle phase transition 0.03991 Highly Informative Inherited
Biological Process (BP) cell morphogenesis involved in differentiation 0.03973 Highly Informative Inherited
Molecular Function (MF) binding 0.0000001043 Least Informative Direct
Molecular Function (MF) protein binding 0.00001898 Moderately Informative Direct
Molecular Function (MF) molecular function regulator 0.00000412 Moderately Informative Direct
Molecular Function (MF) enzyme inhibitor activity 0.0004612 Informative Direct
Molecular Function (MF) enzyme binding 0.02146 Informative Inherited
Molecular Function (MF) protein dimerization activity 0.07314 Informative Inherited
Molecular Function (MF) protein kinase regulator activity 0.000000005511 Highly Informative Direct
Molecular Function (MF) protein heterodimerization activity 0.00000006391 Highly Informative Direct
Cellular Component (CC) cytoplasmic part 0.01537 Least Informative Inherited
Cellular Component (CC) intracellular organelle part 0.7126 Least Informative Inherited
Cellular Component (CC) membrane 0.1544 Least Informative Inherited
Cellular Component (CC) organelle membrane 0.000898 Moderately Informative Direct
Cellular Component (CC) vesicle 0.03326 Moderately Informative Inherited
Cellular Component (CC) cell junction 0.0001066 Informative Direct
Cellular Component (CC) cytoplasmic vesicle part 0.0001236 Informative Direct
Cellular Component (CC) vesicle membrane 0.00006491 Highly Informative Direct

Document: GO annotation of SCOP domains

Disease Ontology (DO)

(show details)
DO termFDR (all)SDDO levelAnnotation (direct or inherited)
Disease Ontology (DO)organ system cancer0Least InformativeDirect
Disease Ontology (DO)nervous system cancer0Moderately InformativeDirect
Disease Ontology (DO)disease of mental health0.001664Moderately InformativeInherited
Disease Ontology (DO)meningioma0InformativeDirect
Disease Ontology (DO)schizophrenia0.00005468InformativeDirect

Document: DO annotation of SCOP domains

Worm Phenotype (WP)

(show details)
WP termFDR (all)SDWP levelAnnotation (direct or inherited)
Worm Phenotype (WP)organism metabolism processing variant0Least InformativeDirect
Worm Phenotype (WP)larval lethal0Least InformativeDirect
Worm Phenotype (WP)retarded heterochronic variations0Least InformativeDirect
Worm Phenotype (WP)cell development variant0Least InformativeDirect
Worm Phenotype (WP)organ system morphology variant0Least InformativeDirect
Worm Phenotype (WP)cell physiology variant0Least InformativeDirect
Worm Phenotype (WP)larval development variant0Least InformativeDirect
Worm Phenotype (WP)larval growth variant0Least InformativeDirect
Worm Phenotype (WP)reproductive system morphology variant0Moderately InformativeDirect
Worm Phenotype (WP)gametogenesis variant0Moderately InformativeDirect
Worm Phenotype (WP)pattern of transgene expression variant0Moderately InformativeDirect
Worm Phenotype (WP)level of transgene expression variant0Moderately InformativeDirect
Worm Phenotype (WP)sterile progeny0Moderately InformativeDirect
Worm Phenotype (WP)protein protein interaction variant0Moderately InformativeDirect
Worm Phenotype (WP)life span variant0InformativeDirect
Worm Phenotype (WP)dauer arrest variant0InformativeDirect
Worm Phenotype (WP)pattern protein expression variant0InformativeDirect
Worm Phenotype (WP)endocytic transport defect0InformativeDirect
Worm Phenotype (WP)oocyte physiology variant0InformativeDirect
Worm Phenotype (WP)enzyme activity reduced0Highly InformativeDirect
Worm Phenotype (WP)protein subcellular localization variant0Highly InformativeDirect
Worm Phenotype (WP)shortened life span0Highly InformativeDirect
Worm Phenotype (WP)transgene expression reduced0Highly InformativeDirect

Document: WP annotation of SCOP domains

Xenopus Anatomy (XA)

(show details)
XA termFDR (all)SDXA levelAnnotation (direct or inherited)
Xenopus ANatomical entity (XAN)embryo0Least InformativeDirect
Xenopus ANatomical entity (XAN)genital system0Least InformativeDirect
Xenopus ANatomical entity (XAN)nervous system0Least InformativeDirect
Xenopus ANatomical entity (XAN)head0Least InformativeDirect
Xenopus ANatomical entity (XAN)tissue0Least InformativeDirect
Xenopus ANatomical entity (XAN)anatomical cluster0Least InformativeDirect
Xenopus ANatomical entity (XAN)ectoderm0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)brain0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)multi-tissue structure0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)embryonic structure0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)sensory system0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)immune system0.02074Moderately InformativeInherited
Xenopus ANatomical entity (XAN)alimentary system0.1082Moderately InformativeInherited
Xenopus ANatomical entity (XAN)skin0InformativeDirect
Xenopus ANatomical entity (XAN)pharyngeal region0InformativeDirect
Xenopus ANatomical entity (XAN)peripheral nervous system0.0000000743InformativeDirect
Xenopus ANatomical entity (XAN)spinal cord0.000001514InformativeDirect
Xenopus ANatomical entity (XAN)endocrine system0.0006011InformativeDirect
Xenopus ANatomical entity (XAN)anatomical space0.001352InformativeInherited
Xenopus ANatomical entity (XAN)ganglion0.0000007296Highly InformativeDirect
Xenopus ANatomical entity (XAN)mesenchyme0.0004106Highly InformativeDirect
Xenopus ANatomical entity (XAN)epidermis0.0006591Highly InformativeDirect

Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details)
AP termFDR (all)SDAP levelAnnotation (direct or inherited)
Plant structure DEvelopment stage (PDE)seed development stage0.0000001061InformativeDirect

Document: AP annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Cellular componentCytoplasm0.000000000002633Least InformativeDirect
Coding sequence diversityAlternative initiation0.000007791Moderately InformativeDirect
Post-translational modificationAcetylation0Least InformativeDirect
Post-translational modificationPhosphoprotein0.00001123Least InformativeDirect
Post-translational modificationNitration0.000000004725InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000308 SSF48445 Protein matches
Abstract

The 14-3-3 proteins are a large family of approximately 30kDa acidic proteins which exist primarily as homo- and heterodimeric within all eukaryotic cells [PubMed1671102, PubMed11911880]. There is a high degree of sequence identity and conservation between all the 14-3-3 isotypes, particularly in the regions which form the dimer interface or line the central ligand binding channel of the dimeric molecule. Each 14-3-3 protein sequence can be roughly divided into three sections: a divergent amino terminus, the conserved core region and a divergent carboxyl terminus. The conserved middle core region of the 14-3-3s encodes an amphipathic groove that forms the main functional domain, a cradle for interacting with client proteins. The monomer consists of nine helices organised in an antiparallel manner, forming an L-shaped structure. The interior of the L-structure is composed of four helices: H3 and H5, which contain many charged and polar amino acids, and H7 and H9, which contain hydrophobic amino acids. These four helices form the concave amphipathic groove that interacts with target peptides.

14-3-3 proteins mainly bind proteins containing phosphothreonine or phosphoserine motifs however exceptions to this rule do exist. Extensive investigation of the 14-3-3 binding site of the mammalian serine/threonine kinase Raf-1 has produced a consensus sequence for 14-3-3-binding, RSxpSxP (in the single-letter amino-acid code, where x denotes any amino acid and p indicates that the next residue is phosphorylated). 14-3-3 proteins appear to effect intracellular signalling in one of three ways - by direct regulation of the catalytic activity of the bound protein, by regulating interactions between the bound protein and other molecules in the cell by sequestration or modification or by controlling the subcellular localisation of the bound ligand. Proteins appear to initially bind to a single dominant site and then subsequently to many, much weaker secondary interaction sites. The 14-3-3 dimer is capable of changing the conformation of its bound ligand whilst itself undergoing minimal structural alteration.


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Worm Phenotype (WP) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 4 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a 14-3-3 protein domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 4 hidden Markov models representing the 14-3-3 protein superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Worm Phenotype (WP) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · Internal database links ]