SUPERFAMILY 1.75 HMM library and genome assignments server

14-3-3 protein superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   All alpha proteins [ 46456] (284)
Fold:   alpha-alpha superhelix [ 48370] (24)
Superfamily:   14-3-3 protein [ 48445]
Families:   14-3-3 protein [ 48446] (4)


Superfamily statistics
Genomes (497) Uniprot 2014_06 PDB chains (SCOP 1.75)
Domains 2,851 3,511 8
Proteins 2,795 3,479 8


Functional annotation
General category coiled coil
Detailed category This is a complex coiled arrangement. The details of which will appear on this page shortly (some coiled coil details are being checked before they are included on the site). If you want to see examples of the states please click here here. If you require further details urgently please contact Owen Rackham

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-quality)

(show details)
GO termFDR (singleton)FDR (all)SDFO levelAnnotation (direct or inherited)
Biological Process (BP)developmental process0.000000021380.0002553Least InformativeDirect
Biological Process (BP)localization0.00000031540.000001159Least InformativeDirect
Biological Process (BP)positive regulation of biological process11Least InformativeInherited
Biological Process (BP)response to stimulus0.038610.03008Least InformativeInherited
Biological Process (BP)regulation of metabolic process0.21080.4827Least InformativeInherited
Biological Process (BP)regulation of cellular process0.30280.8765Least InformativeInherited
Biological Process (BP)single-organism cellular process0.28030.185Least InformativeInherited
Biological Process (BP)multicellular organismal process0.0000000061740.00416Least InformativeInherited
Biological Process (BP)cell differentiation0.00000000090980.00004187Moderately InformativeDirect
Biological Process (BP)macromolecule localization0.000000000072710.000000003238Moderately InformativeDirect
Biological Process (BP)regulation of phosphorus metabolic process0.00044710.0002654Moderately InformativeDirect
Biological Process (BP)cellular localization0.00000032520.000001748Moderately InformativeDirect
Biological Process (BP)organic substance transport0.000016860.000002468Moderately InformativeDirect
Biological Process (BP)regulation of signal transduction0.24560.4444Moderately InformativeInherited
Biological Process (BP)regulation of localization0.30960.2601Moderately InformativeInherited
Biological Process (BP)regulation of cellular component organization0.17620.1374Moderately InformativeInherited
Biological Process (BP)signal transduction0.72030.5712Moderately InformativeInherited
Biological Process (BP)negative regulation of metabolic process0.0073810.002207Moderately InformativeInherited
Biological Process (BP)regulation of catalytic activity0.43840.02419Moderately InformativeInherited
Biological Process (BP)regulation of developmental process0.093780.6211Moderately InformativeInherited
Biological Process (BP)regulation of multicellular organismal process0.22640.815Moderately InformativeInherited
Biological Process (BP)regulation of protein metabolic process0.0037820.00008134Moderately InformativeInherited
Biological Process (BP)negative regulation of cellular process0.02580.01042Moderately InformativeInherited
Biological Process (BP)nervous system development0.0015950.009974Moderately InformativeInherited
Biological Process (BP)organ development0.23130.6836Moderately InformativeInherited
Biological Process (BP)positive regulation of cellular process0.90191Moderately InformativeInherited
Biological Process (BP)positive regulation of response to stimulus0.027160.001416Moderately InformativeInherited
Biological Process (BP)protein transport0.000012150.0001856InformativeDirect
Biological Process (BP)regulation of intracellular transport0.0003190.0002021InformativeDirect
Biological Process (BP)regulation of protein localization0.0005980.000102InformativeDirect
Biological Process (BP)generation of neurons0.000000000082460.00002105InformativeDirect
Biological Process (BP)positive regulation of transport0.00036020.0006011InformativeDirect
Biological Process (BP)single-organism intracellular transport0.0000064840.000003436InformativeDirect
Biological Process (BP)positive regulation of cell death0.0048050.01039InformativeInherited
Biological Process (BP)regulation of apoptotic process0.00079150.001786InformativeInherited
Biological Process (BP)intracellular signal transduction0.054920.001487InformativeInherited
Biological Process (BP)apoptotic process0.000050630.001574InformativeInherited
Biological Process (BP)regulation of protein phosphorylation0.77831InformativeInherited
Biological Process (BP)regulation of transferase activity0.00084830.002036InformativeInherited
Biological Process (BP)regulation of nervous system development0.036380.1121InformativeInherited
Biological Process (BP)regulation of cell development0.24750.3114InformativeInherited
Biological Process (BP)negative regulation of cellular protein metabolic process0.0036410.000002965InformativeInherited
Biological Process (BP)central nervous system development0.00073750.005307InformativeInherited
Biological Process (BP)positive regulation of cellular component organization0.0013410.002994InformativeInherited
Biological Process (BP)regulation of organelle organization0.039430.01678InformativeInherited
Biological Process (BP)cellular component movement0.00056660.03106InformativeInherited
Biological Process (BP)locomotion0.000015590.007895InformativeInherited
Biological Process (BP)localization of cell0.0091320.1538InformativeInherited
Biological Process (BP)positive regulation of organelle organization0.000026180.0002003Highly InformativeDirect
Biological Process (BP)regulation of mitochondrion organization0.0000000015290.0000097Highly InformativeDirect
Biological Process (BP)telencephalon development0.000046240.0001583Highly InformativeDirect
Biological Process (BP)positive regulation of intracellular transport0.0000000028380.0000000445Highly InformativeDirect
Biological Process (BP)intrinsic apoptotic signaling pathway0.000000032530.0003513Highly InformativeDirect
Biological Process (BP)positive regulation of apoptotic signaling pathway0.0000039330.00002793Highly InformativeDirect
Molecular Function (MF)binding00.000000000008128Least InformativeDirect
Molecular Function (MF)protein domain specific binding0.000000000000046370.00000000001258Highly InformativeDirect
Cellular Component (CC)intracellular organelle part0.53510.8668Least InformativeInherited
Cellular Component (CC)membrane0.028880.09301Least InformativeInherited
Cellular Component (CC)cytoplasmic part0.020780.0007833Least InformativeInherited
Cellular Component (CC)intracellular membrane-bounded organelle0.000000006410.004732Least InformativeInherited
Cellular Component (CC)vesicle0.0052720.007816Moderately InformativeInherited
Cellular Component (CC)cell junction0.0000014560.00008469InformativeDirect
Cellular Component (CC)cytoplasmic vesicle membrane0.000014460.0001945Highly InformativeDirect

Document: GO annotation of SCOP domains

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) developmental process 0.0002553 Least Informative Direct
Biological Process (BP) localization 0.000001159 Least Informative Direct
Biological Process (BP) positive regulation of biological process 1 Least Informative Inherited
Biological Process (BP) response to stimulus 0.03008 Least Informative Inherited
Biological Process (BP) regulation of metabolic process 0.4827 Least Informative Inherited
Biological Process (BP) regulation of cellular process 0.8765 Least Informative Inherited
Biological Process (BP) single-organism cellular process 0.185 Least Informative Inherited
Biological Process (BP) multicellular organismal process 0.00416 Least Informative Inherited
Biological Process (BP) cell differentiation 0.00004187 Moderately Informative Direct
Biological Process (BP) macromolecule localization 0.000000003238 Moderately Informative Direct
Biological Process (BP) regulation of phosphorus metabolic process 0.0002654 Moderately Informative Direct
Biological Process (BP) regulation of protein metabolic process 0.00008134 Moderately Informative Direct
Biological Process (BP) cellular localization 0.000001748 Moderately Informative Direct
Biological Process (BP) organic substance transport 0.000002468 Moderately Informative Direct
Biological Process (BP) regulation of signal transduction 0.4444 Moderately Informative Inherited
Biological Process (BP) regulation of localization 0.2601 Moderately Informative Inherited
Biological Process (BP) regulation of cellular component organization 0.1374 Moderately Informative Inherited
Biological Process (BP) signal transduction 0.5712 Moderately Informative Inherited
Biological Process (BP) negative regulation of metabolic process 0.002207 Moderately Informative Inherited
Biological Process (BP) regulation of catalytic activity 0.02419 Moderately Informative Inherited
Biological Process (BP) regulation of developmental process 0.6211 Moderately Informative Inherited
Biological Process (BP) regulation of multicellular organismal process 0.815 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.01042 Moderately Informative Inherited
Biological Process (BP) nervous system development 0.009974 Moderately Informative Inherited
Biological Process (BP) organ development 0.6836 Moderately Informative Inherited
Biological Process (BP) positive regulation of cellular process 1 Moderately Informative Inherited
Biological Process (BP) positive regulation of response to stimulus 0.001416 Moderately Informative Inherited
Biological Process (BP) cellular response to stress 0.0143 Moderately Informative Inherited
Biological Process (BP) protein transport 0.0001856 Informative Direct
Biological Process (BP) negative regulation of cellular protein metabolic process 0.000002965 Informative Direct
Biological Process (BP) regulation of intracellular transport 0.0002021 Informative Direct
Biological Process (BP) regulation of protein localization 0.000102 Informative Direct
Biological Process (BP) generation of neurons 0.00002105 Informative Direct
Biological Process (BP) positive regulation of transport 0.0006011 Informative Direct
Biological Process (BP) single-organism intracellular transport 0.000003436 Informative Direct
Biological Process (BP) positive regulation of cell death 0.01039 Informative Inherited
Biological Process (BP) regulation of apoptotic process 0.001786 Informative Inherited
Biological Process (BP) intracellular signal transduction 0.001487 Informative Inherited
Biological Process (BP) apoptotic process 0.001574 Informative Inherited
Biological Process (BP) regulation of protein phosphorylation 1 Informative Inherited
Biological Process (BP) regulation of transferase activity 0.002036 Informative Inherited
Biological Process (BP) regulation of nervous system development 0.1121 Informative Inherited
Biological Process (BP) regulation of cell development 0.3114 Informative Inherited
Biological Process (BP) cell cycle process 0.002802 Informative Inherited
Biological Process (BP) central nervous system development 0.005307 Informative Inherited
Biological Process (BP) positive regulation of cellular component organization 0.002994 Informative Inherited
Biological Process (BP) regulation of organelle organization 0.01678 Informative Inherited
Biological Process (BP) cellular component movement 0.03106 Informative Inherited
Biological Process (BP) locomotion 0.007895 Informative Inherited
Biological Process (BP) localization of cell 0.1538 Informative Inherited
Biological Process (BP) positive regulation of organelle organization 0.0002003 Highly Informative Direct
Biological Process (BP) regulation of mitochondrion organization 0.0000097 Highly Informative Direct
Biological Process (BP) telencephalon development 0.0001583 Highly Informative Direct
Biological Process (BP) DNA integrity checkpoint 0.00000004688 Highly Informative Direct
Biological Process (BP) positive regulation of intracellular transport 0.0000000445 Highly Informative Direct
Biological Process (BP) intrinsic apoptotic signaling pathway 0.0003513 Highly Informative Direct
Biological Process (BP) positive regulation of apoptotic signaling pathway 0.00002793 Highly Informative Direct
Molecular Function (MF) binding 0.000000000008128 Least Informative Direct
Molecular Function (MF) enzyme regulator activity 0.0004153 Moderately Informative Direct
Molecular Function (MF) protein domain specific binding 0.00000000001258 Highly Informative Direct
Cellular Component (CC) cytoplasmic part 0.0007833 Least Informative Direct
Cellular Component (CC) intracellular organelle part 0.8668 Least Informative Inherited
Cellular Component (CC) membrane 0.09301 Least Informative Inherited
Cellular Component (CC) intracellular membrane-bounded organelle 0.004732 Least Informative Inherited
Cellular Component (CC) vesicle 0.007816 Moderately Informative Inherited
Cellular Component (CC) cell junction 0.00008469 Informative Direct
Cellular Component (CC) cytoplasmic vesicle membrane 0.0001945 Highly Informative Direct

Document: GO annotation of SCOP domains

Disease Ontology (DO)

(show details)
DO termFDR (all)SDDO levelAnnotation (direct or inherited)
Disease Ontology (DO)organ system cancer0Least InformativeDirect
Disease Ontology (DO)nervous system cancer0Moderately InformativeDirect
Disease Ontology (DO)disease of mental health0.003373Moderately InformativeInherited
Disease Ontology (DO)meningioma0InformativeDirect
Disease Ontology (DO)schizophrenia0.0000601InformativeDirect

Document: DO annotation of SCOP domains

Xenopus Anatomy (XA)

(show details)
XA termFDR (all)SDXA levelAnnotation (direct or inherited)
Xenopus ANatomical entity (XAN)embryo0Least InformativeDirect
Xenopus ANatomical entity (XAN)genital system0Least InformativeDirect
Xenopus ANatomical entity (XAN)central nervous system0Least InformativeDirect
Xenopus ANatomical entity (XAN)head0Least InformativeDirect
Xenopus ANatomical entity (XAN)viscus0Least InformativeDirect
Xenopus ANatomical entity (XAN)portion of tissue0Least InformativeDirect
Xenopus ANatomical entity (XAN)ectoderm0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)surface structure0Moderately InformativeDirect
Xenopus ANatomical entity (XAN)embryonic structure0.004126Moderately InformativeInherited
Xenopus ANatomical entity (XAN)skin0InformativeDirect
Xenopus ANatomical entity (XAN)marginal zone0.000269Highly InformativeDirect

Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details)
AP termFDR (all)SDAP levelAnnotation (direct or inherited)
Plant structure DEvelopment stage (PDE)seed development stage0.0000001059InformativeDirect

Document: AP annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Cellular componentCytoplasm0.000000000006158Least InformativeDirect
Coding sequence diversityAlternative initiation0.000007286Moderately InformativeDirect
Post-translational modificationAcetylation0Least InformativeDirect
Post-translational modificationPhosphoprotein0.000001037Least InformativeDirect
Post-translational modificationNitration0.000000003446InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000308 SSF48445 Protein matches
Abstract

The 14-3-3 proteins are a large family of approximately 30kDa acidic proteins which exist primarily as homo- and heterodimeric within all eukaryotic cells [PubMed1671102, PubMed11911880]. There is a high degree of sequence identity and conservation between all the 14-3-3 isotypes, particularly in the regions which form the dimer interface or line the central ligand binding channel of the dimeric molecule. Each 14-3-3 protein sequence can be roughly divided into three sections: a divergent amino terminus, the conserved core region and a divergent carboxyl terminus. The conserved middle core region of the 14-3-3s encodes an amphipathic groove that forms the main functional domain, a cradle for interacting with client proteins. The monomer consists of nine helices organised in an antiparallel manner, forming an L-shaped structure. The interior of the L-structure is composed of four helices: H3 and H5, which contain many charged and polar amino acids, and H7 and H9, which contain hydrophobic amino acids. These four helices form the concave amphipathic groove that interacts with target peptides.

14-3-3 proteins mainly bind proteins containing phosphothreonine or phosphoserine motifs however exceptions to this rule do exist. Extensive investigation of the 14-3-3 binding site of the mammalian serine/threonine kinase Raf-1 has produced a consensus sequence for 14-3-3-binding, RSxpSxP (in the single-letter amino-acid code, where x denotes any amino acid and p indicates that the next residue is phosphorylated). 14-3-3 proteins appear to effect intracellular signalling in one of three ways - by direct regulation of the catalytic activity of the bound protein, by regulating interactions between the bound protein and other molecules in the cell by sequestration or modification or by controlling the subcellular localisation of the bound ligand. Proteins appear to initially bind to a single dominant site and then subsequently to many, much weaker secondary interaction sites. The 14-3-3 dimer is capable of changing the conformation of its bound ligand whilst itself undergoing minimal structural alteration.


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 4 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a 14-3-3 protein domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 4 hidden Markov models representing the 14-3-3 protein superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Disease Ontology (DO) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · Internal database links ]