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14-3-3 protein superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   All alpha proteins [ 46456] (284)
Fold:   alpha-alpha superhelix [ 48370] (24)
Superfamily:   14-3-3 protein [ 48445]
Families:   14-3-3 protein [ 48446] (4)


Superfamily statistics
Genomes (505) Uniprot 2017_06 genome PDB chains (SCOP 1.75)
Domains 2,955 6,867 8
Proteins 2,896 6,755 8


Functional annotation
General category coiled coil
Detailed category This is a complex coiled arrangement. The details of which will appear on this page shortly (some coiled coil details are being checked before they are included on the site). If you want to see examples of the states please click here here. If you require further details urgently please contact Owen Rackham

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-quality)

(show details)
GO termFDR (singleton)FDR (all)SDFO levelAnnotation (direct or inherited)
Biological Process (BP)biological regulation0.0000000000001280.0000034Least InformativeDirect
Biological Process (BP)single-organism cellular process0.10570.2257Least InformativeInherited
Biological Process (BP)multicellular organismal process0.00000014860.03926Least InformativeInherited
Biological Process (BP)cellular component organization or biogenesis0.000011640.01276Least InformativeInherited
Biological Process (BP)response to stimulus0.00071410.001382Least InformativeInherited
Biological Process (BP)reproduction0.00008220.000435Moderately InformativeDirect
Biological Process (BP)cell communication0.00000013070.0002057Moderately InformativeDirect
Biological Process (BP)single organism signaling0.0000027340.0002902Moderately InformativeDirect
Biological Process (BP)regulation of localization0.35140.2765Moderately InformativeInherited
Biological Process (BP)tissue development0.25310.5651Moderately InformativeInherited
Biological Process (BP)cellular response to chemical stimulus0.48660.782Moderately InformativeInherited
Biological Process (BP)response to endogenous stimulus0.01770.1447Moderately InformativeInherited
Biological Process (BP)response to oxygen-containing compound0.26240.3446Moderately InformativeInherited
Biological Process (BP)anatomical structure morphogenesis0.48660.5978Moderately InformativeInherited
Biological Process (BP)response to organic substance0.026790.5178Moderately InformativeInherited
Biological Process (BP)cell differentiation0.00000013270.001075Moderately InformativeInherited
Biological Process (BP)response to abiotic stimulus0.28650.4504Moderately InformativeInherited
Biological Process (BP)macromolecule localization0.016850.0183Moderately InformativeInherited
Biological Process (BP)response to temperature stimulus0.000043930.0003056InformativeDirect
Biological Process (BP)response to organic cyclic compound0.000026110.00002541InformativeDirect
Biological Process (BP)cellular response to hormone stimulus0.0000011780.0000116InformativeDirect
Biological Process (BP)growth0.00019020.00006258InformativeDirect
Biological Process (BP)single organism reproductive process0.000087250.0004145InformativeDirect
Biological Process (BP)cellular response to lipid0.00028440.00004169InformativeDirect
Biological Process (BP)cellular response to oxygen-containing compound0.00059590.0006537InformativeDirect
Biological Process (BP)regulation of protein localization0.010920.003596InformativeInherited
Biological Process (BP)regulation of cellular localization0.0023250.001474InformativeInherited
Biological Process (BP)cell development0.037090.0245InformativeInherited
Biological Process (BP)reproductive system development0.0056770.004941InformativeInherited
Biological Process (BP)post-embryonic development0.0032980.001584InformativeInherited
Biological Process (BP)multicellular organism reproduction0.01080.01197InformativeInherited
Biological Process (BP)developmental process involved in reproduction0.0095570.001845InformativeInherited
Biological Process (BP)cellular component morphogenesis0.0043910.0391InformativeInherited
Biological Process (BP)hormone-mediated signaling pathway0.000057360.00002279Highly InformativeDirect
Biological Process (BP)trichome differentiation0.0000098870.00000006512Highly InformativeDirect
Biological Process (BP)cell growth0.00000028840.00000008267Highly InformativeDirect
Biological Process (BP)seed development0.0000081940.000002493Highly InformativeDirect
Biological Process (BP)regulation of cell division0.00012780.00001705Highly InformativeDirect
Biological Process (BP)developmental growth involved in morphogenesis0.0000016970.00002211Highly InformativeDirect
Biological Process (BP)cellular response to steroid hormone stimulus0.000016290.000001631Highly InformativeDirect
Biological Process (BP)positive regulation of cellular protein localization0.0000047670.00002641Highly InformativeDirect
Biological Process (BP)cell morphogenesis involved in differentiation0.0023110.04075Highly InformativeInherited
Molecular Function (MF)binding0.000000000000043090.00000001465Least InformativeDirect
Molecular Function (MF)molecular function regulator0.000012280.000007667Moderately InformativeDirect
Molecular Function (MF)enzyme binding0.00050560.01599InformativeInherited
Molecular Function (MF)protein dimerization activity0.21210.1593InformativeInherited
Molecular Function (MF)enzyme inhibitor activity0.049310.0004125InformativeInherited
Molecular Function (MF)kinase regulator activity0.000017340.0001216Highly InformativeDirect
Molecular Function (MF)protein domain specific binding0.00000000024180.0000008618Highly InformativeDirect
Molecular Function (MF)protein heterodimerization activity0.000000001150.0000001737Highly InformativeDirect
Cellular Component (CC)cytoplasmic part0.23610.01084Least InformativeInherited
Cellular Component (CC)intracellular organelle part0.56730.9698Least InformativeInherited
Cellular Component (CC)membrane0.067130.1137Least InformativeInherited
Cellular Component (CC)vesicle0.019740.07074Moderately InformativeInherited
Cellular Component (CC)cytoplasmic vesicle part0.000065070.001007InformativeInherited
Cellular Component (CC)cytoplasmic vesicle membrane0.000000020750.000004527Highly InformativeDirect

Document: GO annotation of SCOP domains

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) biological regulation 0.0000034 Least Informative Direct
Biological Process (BP) multicellular organismal process 0.03926 Least Informative Inherited
Biological Process (BP) cellular component organization or biogenesis 0.01276 Least Informative Inherited
Biological Process (BP) response to stimulus 0.001382 Least Informative Inherited
Biological Process (BP) single-organism cellular process 0.2257 Least Informative Inherited
Biological Process (BP) reproduction 0.000435 Moderately Informative Direct
Biological Process (BP) cell communication 0.0002057 Moderately Informative Direct
Biological Process (BP) single organism signaling 0.0002902 Moderately Informative Direct
Biological Process (BP) regulation of localization 0.2765 Moderately Informative Inherited
Biological Process (BP) tissue development 0.5651 Moderately Informative Inherited
Biological Process (BP) cellular response to chemical stimulus 0.782 Moderately Informative Inherited
Biological Process (BP) response to oxygen-containing compound 0.3446 Moderately Informative Inherited
Biological Process (BP) anatomical structure morphogenesis 0.5978 Moderately Informative Inherited
Biological Process (BP) response to endogenous stimulus 0.1447 Moderately Informative Inherited
Biological Process (BP) response to organic substance 0.5178 Moderately Informative Inherited
Biological Process (BP) cell differentiation 0.001075 Moderately Informative Inherited
Biological Process (BP) response to abiotic stimulus 0.4504 Moderately Informative Inherited
Biological Process (BP) macromolecule localization 0.0183 Moderately Informative Inherited
Biological Process (BP) organelle organization 0.8682 Moderately Informative Inherited
Biological Process (BP) cellular localization 0.01827 Moderately Informative Inherited
Biological Process (BP) cellular response to stress 0.1098 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.3369 Moderately Informative Inherited
Biological Process (BP) mitochondrion organization 0.0002145 Informative Direct
Biological Process (BP) response to temperature stimulus 0.0003056 Informative Direct
Biological Process (BP) response to organic cyclic compound 0.00002541 Informative Direct
Biological Process (BP) cell cycle process 0.0003829 Informative Direct
Biological Process (BP) cellular response to hormone stimulus 0.0000116 Informative Direct
Biological Process (BP) growth 0.00006258 Informative Direct
Biological Process (BP) single organism reproductive process 0.0004145 Informative Direct
Biological Process (BP) regulation of cell cycle 0.0003691 Informative Direct
Biological Process (BP) cellular response to lipid 0.00004169 Informative Direct
Biological Process (BP) cellular response to oxygen-containing compound 0.0006537 Informative Direct
Biological Process (BP) regulation of protein localization 0.003596 Informative Inherited
Biological Process (BP) regulation of cellular localization 0.001474 Informative Inherited
Biological Process (BP) cell development 0.0245 Informative Inherited
Biological Process (BP) reproductive system development 0.004941 Informative Inherited
Biological Process (BP) post-embryonic development 0.001584 Informative Inherited
Biological Process (BP) multicellular organism reproduction 0.01197 Informative Inherited
Biological Process (BP) developmental process involved in reproduction 0.001845 Informative Inherited
Biological Process (BP) cellular component morphogenesis 0.0391 Informative Inherited
Biological Process (BP) intracellular transport 0.005033 Informative Inherited
Biological Process (BP) cellular response to DNA damage stimulus 0.05303 Informative Inherited
Biological Process (BP) cell cycle checkpoint 0.00000003534 Highly Informative Direct
Biological Process (BP) mitochondrial transport 0.0002397 Highly Informative Direct
Biological Process (BP) hormone-mediated signaling pathway 0.00002279 Highly Informative Direct
Biological Process (BP) trichome differentiation 0.00000006512 Highly Informative Direct
Biological Process (BP) cell growth 0.00000008267 Highly Informative Direct
Biological Process (BP) seed development 0.000002493 Highly Informative Direct
Biological Process (BP) regulation of cell division 0.00001705 Highly Informative Direct
Biological Process (BP) developmental growth involved in morphogenesis 0.00002211 Highly Informative Direct
Biological Process (BP) cellular response to steroid hormone stimulus 0.000001631 Highly Informative Direct
Biological Process (BP) negative regulation of cell cycle phase transition 0.000002338 Highly Informative Direct
Biological Process (BP) positive regulation of cellular protein localization 0.00002641 Highly Informative Direct
Biological Process (BP) negative regulation of mitotic cell cycle 0.04782 Highly Informative Inherited
Biological Process (BP) regulation of mitotic cell cycle phase transition 0.04496 Highly Informative Inherited
Biological Process (BP) cell morphogenesis involved in differentiation 0.04075 Highly Informative Inherited
Molecular Function (MF) binding 0.00000001465 Least Informative Direct
Molecular Function (MF) molecular function regulator 0.000007667 Moderately Informative Direct
Molecular Function (MF) enzyme inhibitor activity 0.0004125 Informative Direct
Molecular Function (MF) enzyme binding 0.01599 Informative Inherited
Molecular Function (MF) protein dimerization activity 0.1593 Informative Inherited
Molecular Function (MF) kinase regulator activity 0.0001216 Highly Informative Direct
Molecular Function (MF) protein domain specific binding 0.0000008618 Highly Informative Direct
Molecular Function (MF) protein heterodimerization activity 0.0000001737 Highly Informative Direct
Cellular Component (CC) intracellular organelle part 0.9698 Least Informative Inherited
Cellular Component (CC) membrane 0.1137 Least Informative Inherited
Cellular Component (CC) cytoplasmic part 0.01084 Least Informative Inherited
Cellular Component (CC) vesicle 0.07074 Moderately Informative Inherited
Cellular Component (CC) external encapsulating structure 0.0006307 Informative Direct
Cellular Component (CC) cytoplasmic vesicle part 0.001007 Informative Inherited
Cellular Component (CC) cytoplasmic vesicle membrane 0.000004527 Highly Informative Direct

Document: GO annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Cellular componentCytoplasm0Least InformativeDirect
Cellular componentNucleus0.0000001097Least InformativeDirect
Coding sequence diversityAlternative initiation0.000008251Moderately InformativeDirect
Post-translational modificationAcetylation0.00000000000001676Least InformativeDirect
Post-translational modificationPhosphoprotein0.000001354Least InformativeDirect
Post-translational modificationUbl conjugation0.0001126Moderately InformativeDirect
Post-translational modificationIsopeptide bond0.0003013Moderately InformativeDirect
Post-translational modificationNitration4.894e-16InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000308 SSF48445 Protein matches
Abstract

The 14-3-3 proteins are a large family of approximately 30kDa acidic proteins which exist primarily as homo- and heterodimeric within all eukaryotic cells [PubMed1671102, PubMed11911880]. There is a high degree of sequence identity and conservation between all the 14-3-3 isotypes, particularly in the regions which form the dimer interface or line the central ligand binding channel of the dimeric molecule. Each 14-3-3 protein sequence can be roughly divided into three sections: a divergent amino terminus, the conserved core region and a divergent carboxyl terminus. The conserved middle core region of the 14-3-3s encodes an amphipathic groove that forms the main functional domain, a cradle for interacting with client proteins. The monomer consists of nine helices organised in an antiparallel manner, forming an L-shaped structure. The interior of the L-structure is composed of four helices: H3 and H5, which contain many charged and polar amino acids, and H7 and H9, which contain hydrophobic amino acids. These four helices form the concave amphipathic groove that interacts with target peptides.

14-3-3 proteins mainly bind proteins containing phosphothreonine or phosphoserine motifs however exceptions to this rule do exist. Extensive investigation of the 14-3-3 binding site of the mammalian serine/threonine kinase Raf-1 has produced a consensus sequence for 14-3-3-binding, RSxpSxP (in the single-letter amino-acid code, where x denotes any amino acid and p indicates that the next residue is phosphorylated). 14-3-3 proteins appear to effect intracellular signalling in one of three ways - by direct regulation of the catalytic activity of the bound protein, by regulating interactions between the bound protein and other molecules in the cell by sequestration or modification or by controlling the subcellular localisation of the bound ligand. Proteins appear to initially bind to a single dominant site and then subsequently to many, much weaker secondary interaction sites. The 14-3-3 dimer is capable of changing the conformation of its bound ligand whilst itself undergoing minimal structural alteration.


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 4 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a 14-3-3 protein domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 4 hidden Markov models representing the 14-3-3 protein superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · UniProtKB KeyWords (KW) · Internal database links ]