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ENTH/VHS domain superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   All alpha proteins [ 46456] (284)
Fold:   alpha-alpha superhelix [ 48370] (24)
Superfamily:   ENTH/VHS domain [ 48464] (4)
Families:   ENTH domain [ 48465]
  VHS domain [ 48468] (5)
  Phosphoinositide-binding clathrin adaptor, N-terminal domain [ 100911] (2)
  RPR domain (SMART 00582 ) [ 109975]


Superfamily statistics
Genomes (499) Uniprot 2014_06 PDB chains (SCOP 1.75)
Domains 9,713 9,644 14
Proteins 9,680 9,623 14


Functional annotation
General category Processes_IC
Detailed category Transport

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) cellular component organization or biogenesis 0.3778 Least Informative Inherited
Biological Process (BP) biological regulation 0.4049 Least Informative Inherited
Biological Process (BP) cellular nitrogen compound metabolic process 1 Least Informative Inherited
Biological Process (BP) macromolecule metabolic process 0.5831 Least Informative Inherited
Biological Process (BP) heterocycle metabolic process 0.8678 Least Informative Inherited
Biological Process (BP) single-organism cellular process 0.9726 Least Informative Inherited
Biological Process (BP) primary metabolic process 1 Least Informative Inherited
Biological Process (BP) organic cyclic compound metabolic process 0.9969 Least Informative Inherited
Biological Process (BP) cellular aromatic compound metabolic process 0.8869 Least Informative Inherited
Biological Process (BP) biosynthetic process 1 Least Informative Inherited
Biological Process (BP) cellular localization 0.000005763 Moderately Informative Direct
Biological Process (BP) cellular component assembly 0.0006159 Moderately Informative Direct
Biological Process (BP) regulation of cell communication 0.01364 Moderately Informative Inherited
Biological Process (BP) cell communication 0.02182 Moderately Informative Inherited
Biological Process (BP) macromolecule localization 0.00832 Moderately Informative Inherited
Biological Process (BP) cellular protein modification process 1 Moderately Informative Inherited
Biological Process (BP) regulation of gene expression 1 Moderately Informative Inherited
Biological Process (BP) regulation of nitrogen compound metabolic process 1 Moderately Informative Inherited
Biological Process (BP) regulation of biosynthetic process 1 Moderately Informative Inherited
Biological Process (BP) organic substance transport 0.8308 Moderately Informative Inherited
Biological Process (BP) regulation of localization 0.02754 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.07909 Moderately Informative Inherited
Biological Process (BP) single-organism transport 1 Moderately Informative Inherited
Biological Process (BP) regulation of response to stimulus 0.1243 Moderately Informative Inherited
Biological Process (BP) cellular catabolic process 0.6543 Moderately Informative Inherited
Biological Process (BP) organic substance catabolic process 0.8653 Moderately Informative Inherited
Biological Process (BP) regulation of multicellular organismal process 0.589 Moderately Informative Inherited
Biological Process (BP) macromolecular complex subunit organization 0.448 Moderately Informative Inherited
Biological Process (BP) gene expression 0.07252 Moderately Informative Inherited
Biological Process (BP) regulation of cellular component organization 0.1512 Moderately Informative Inherited
Biological Process (BP) regulation of cellular metabolic process 1 Moderately Informative Inherited
Biological Process (BP) positive regulation of cellular process 0.04278 Moderately Informative Inherited
Biological Process (BP) regulation of protein metabolic process 0.2287 Moderately Informative Inherited
Biological Process (BP) regulation of molecular function 0.6726 Moderately Informative Inherited
Biological Process (BP) positive regulation of metabolic process 0.9752 Moderately Informative Inherited
Biological Process (BP) regulation of developmental process 0.09072 Moderately Informative Inherited
Biological Process (BP) regulation of signaling 0.01347 Moderately Informative Inherited
Biological Process (BP) regulation of biological quality 0.009609 Moderately Informative Inherited
Biological Process (BP) signaling 0.006456 Moderately Informative Inherited
Biological Process (BP) organelle organization 0.05306 Moderately Informative Inherited
Biological Process (BP) RNA metabolic process 0.02302 Moderately Informative Inherited
Biological Process (BP) nucleobase-containing compound biosynthetic process 0.1225 Moderately Informative Inherited
Biological Process (BP) cellular macromolecule biosynthetic process 0.7207 Moderately Informative Inherited
Biological Process (BP) vesicle-mediated transport 0.00000003288 Informative Direct
Biological Process (BP) secretion 0.00000008523 Informative Direct
Biological Process (BP) cellular macromolecular complex assembly 0.0001867 Informative Direct
Biological Process (BP) positive regulation of signal transduction 0.000345 Informative Direct
Biological Process (BP) actin filament-based process 0.0009135 Informative Direct
Biological Process (BP) negative regulation of multicellular organismal process 0.01258 Informative Inherited
Biological Process (BP) negative regulation of signaling 0.2103 Informative Inherited
Biological Process (BP) negative regulation of cell communication 0.2843 Informative Inherited
Biological Process (BP) negative regulation of response to stimulus 0.3586 Informative Inherited
Biological Process (BP) protein localization to organelle 1 Informative Inherited
Biological Process (BP) regulation of transcription, DNA-templated 1 Informative Inherited
Biological Process (BP) transmembrane transport 0.2928 Informative Inherited
Biological Process (BP) regulation of cell differentiation 0.2056 Informative Inherited
Biological Process (BP) cellular macromolecule catabolic process 0.03338 Informative Inherited
Biological Process (BP) proteolysis 0.1817 Informative Inherited
Biological Process (BP) system process 0.3405 Informative Inherited
Biological Process (BP) single-organism intracellular transport 0.001528 Informative Inherited
Biological Process (BP) regulation of proteolysis 0.05379 Informative Inherited
Biological Process (BP) regulation of hydrolase activity 0.07324 Informative Inherited
Biological Process (BP) positive regulation of catalytic activity 0.07431 Informative Inherited
Biological Process (BP) positive regulation of cellular protein metabolic process 0.2108 Informative Inherited
Biological Process (BP) cytoskeleton organization 0.1605 Informative Inherited
Biological Process (BP) protein targeting 1 Informative Inherited
Biological Process (BP) transcription, DNA-templated 0.00415 Informative Inherited
Biological Process (BP) vesicle localization 0 Highly Informative Direct
Biological Process (BP) regulation of neurotransmitter levels 0 Highly Informative Direct
Biological Process (BP) synaptic signaling 0.00000000000003559 Highly Informative Direct
Biological Process (BP) signal release 0.000000000001055 Highly Informative Direct
Biological Process (BP) autophagy 0.000000000006791 Highly Informative Direct
Biological Process (BP) endosomal transport 0.000000008335 Highly Informative Direct
Biological Process (BP) regulation of vesicle-mediated transport 0.00000005912 Highly Informative Direct
Biological Process (BP) vesicle organization 0.000000389 Highly Informative Direct
Biological Process (BP) actin filament organization 0.000006027 Highly Informative Direct
Biological Process (BP) establishment of organelle localization 0.00006487 Highly Informative Direct
Biological Process (BP) transcription from RNA polymerase II promoter 0.0001101 Highly Informative Direct
Biological Process (BP) mRNA metabolic process 0.0001229 Highly Informative Direct
Biological Process (BP) endocytosis 0.0003099 Highly Informative Direct
Biological Process (BP) protein dephosphorylation 0.0008173 Highly Informative Direct
Biological Process (BP) regulation of transcription from RNA polymerase II promoter 0.7961 Highly Informative Inherited
Biological Process (BP) protein import 0.001674 Highly Informative Inherited
Biological Process (BP) modification-dependent protein catabolic process 0.007804 Highly Informative Inherited
Biological Process (BP) sensory perception 0.02222 Highly Informative Inherited
Biological Process (BP) positive regulation of proteolysis 0.002032 Highly Informative Inherited
Biological Process (BP) Golgi vesicle transport 0.6311 Highly Informative Inherited
Molecular Function (MF) binding 0.0000000458 Least Informative Direct
Molecular Function (MF) anion binding 0.0000008987 Moderately Informative Direct
Molecular Function (MF) protein binding 0.0002351 Moderately Informative Direct
Molecular Function (MF) nucleic acid binding 0.3536 Moderately Informative Inherited
Molecular Function (MF) phospholipid binding 0.000000000000002458 Informative Direct
Molecular Function (MF) RNA binding 0.0002986 Informative Direct
Molecular Function (MF) enzyme binding 0.03131 Informative Inherited
Molecular Function (MF) macromolecular complex binding 0.1869 Informative Inherited
Cellular Component (CC) intracellular organelle part 0.0000000661 Least Informative Direct
Cellular Component (CC) intracellular membrane-bounded organelle 1 Least Informative Inherited
Cellular Component (CC) protein complex 0.1174 Least Informative Inherited
Cellular Component (CC) cytoplasmic part 0.006404 Least Informative Inherited
Cellular Component (CC) membrane 0.00794 Least Informative Inherited
Cellular Component (CC) non-membrane-bounded organelle 1 Least Informative Inherited
Cellular Component (CC) vesicle 0.00000000000004778 Moderately Informative Direct
Cellular Component (CC) whole membrane 0.000000002877 Moderately Informative Direct
Cellular Component (CC) organelle membrane 0.03731 Moderately Informative Inherited
Cellular Component (CC) plasma membrane 0.964 Moderately Informative Inherited
Cellular Component (CC) nuclear part 0.1501 Moderately Informative Inherited
Cellular Component (CC) cytoskeleton 0.002395 Moderately Informative Inherited
Cellular Component (CC) membrane protein complex 0.01847 Moderately Informative Inherited
Cellular Component (CC) intracellular organelle lumen 0.01146 Moderately Informative Inherited
Cellular Component (CC) transferase complex 0.05993 Moderately Informative Inherited
Cellular Component (CC) cytoplasmic vesicle part 0.000000000004012 Informative Direct
Cellular Component (CC) membrane region 0.000000005137 Informative Direct
Cellular Component (CC) cytoplasmic region 0.00000007575 Informative Direct
Cellular Component (CC) Golgi apparatus 0.0000008962 Informative Direct
Cellular Component (CC) DNA-directed RNA polymerase complex 0.000003217 Informative Direct
Cellular Component (CC) organelle subcompartment 0.0001175 Informative Direct
Cellular Component (CC) synapse 0.0001294 Informative Direct
Cellular Component (CC) endosome 0.0002224 Informative Direct
Cellular Component (CC) actin cytoskeleton 0.0008377 Informative Direct
Cellular Component (CC) plasma membrane region 0.1429 Informative Inherited
Cellular Component (CC) nucleoplasm part 0.1199 Informative Inherited
Cellular Component (CC) cortical cytoskeleton 0.0000002265 Highly Informative Direct
Cellular Component (CC) coated vesicle 0.00005051 Highly Informative Direct
Cellular Component (CC) vesicle membrane 0.1084 Highly Informative Inherited
Cellular Component (CC) endocytic vesicle 0.9882 Highly Informative Inherited

Document: GO annotation of SCOP domains

Mouse Phenotype (MP)

(show details)
MP termFDR (all)SDMP levelAnnotation (direct or inherited)
Mammalian Phenotype (MP)growth/size/body region phenotype0.04527Least InformativeInherited
Mammalian Phenotype (MP)abnormal homeostasis0.989Least InformativeInherited
Mammalian Phenotype (MP)abnormal body size0.8532Moderately InformativeInherited
Mammalian Phenotype (MP)decreased total tissue mass0.02214InformativeInherited
Mammalian Phenotype (MP)decreased body size0.06995InformativeInherited
Mammalian Phenotype (MP)abnormal glucose homeostasis0.187InformativeInherited
Mammalian Phenotype (MP)postnatal growth retardation0.000009175Highly InformativeDirect
Mammalian Phenotype (MP)decreased birth weight0.0006411Highly InformativeDirect
Mammalian Phenotype (MP)hypoglycemia0.000793Highly InformativeDirect

Document: MP annotation of SCOP domains

Worm Phenotype (WP)

(show details)
WP termFDR (all)SDWP levelAnnotation (direct or inherited)
Worm Phenotype (WP)larval lethal0Least InformativeDirect
Worm Phenotype (WP)cell physiology variant0.00005469Least InformativeDirect
Worm Phenotype (WP)organism metabolism processing variant0.004673Least InformativeInherited
Worm Phenotype (WP)organism environmental stimulus response variant0.2262Least InformativeInherited
Worm Phenotype (WP)apoptosis variant0.000003626Moderately InformativeDirect
Worm Phenotype (WP)chemical response variant0.01709Moderately InformativeInherited
Worm Phenotype (WP)pattern of transgene expression variant0.398Moderately InformativeInherited
Worm Phenotype (WP)accumulated germline cell corpses0.0000000983InformativeDirect
Worm Phenotype (WP)aldicarb response variant0.0009157InformativeDirect
Worm Phenotype (WP)endocytic transport defect0.008042InformativeInherited
Worm Phenotype (WP)drug resistant0.125InformativeInherited
Worm Phenotype (WP)coelomocyte uptake defective0.000007051Highly InformativeDirect
Worm Phenotype (WP)aldicarb resistant0.0003389Highly InformativeDirect

Document: WP annotation of SCOP domains

Fly Anatomy (FA)

(show details)
FA termFDR (all)SDFA levelAnnotation (direct or inherited)
Fly Anatomy (FA)larva0.2729Least InformativeInherited
Fly Anatomy (FA)cell0.449Least InformativeInherited
Fly Anatomy (FA)multi-tissue structure0.4545Least InformativeInherited
Fly Anatomy (FA)portion of tissue0.07382Moderately InformativeInherited
Fly Anatomy (FA)developing material anatomical entity0.3121Moderately InformativeInherited
Fly Anatomy (FA)epithelial cell0.002798InformativeInherited
Fly Anatomy (FA)imaginal precursor0.01551InformativeInherited
Fly Anatomy (FA)prepupa0.01854InformativeInherited
Fly Anatomy (FA)pupa0.02331InformativeInherited
Fly Anatomy (FA)eye disc0.0009244Highly InformativeDirect

Document: FA annotation of SCOP domains

Zebrafish Anatomy (ZA)

(show details)
ZA termFDR (all)SDZA levelAnnotation (direct or inherited)
Zebrafish Anatomy (ZA)organism subdivision0Least InformativeDirect
Zebrafish Anatomy (ZA)head0Moderately InformativeDirect
Zebrafish Anatomy (ZA)digestive system0InformativeDirect

Document: ZA annotation of SCOP domains

Xenopus Anatomy (XA)

(show details) Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details) Document: AP annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Biological processTransport0Least InformativeDirect
Biological processProtein transport0Moderately InformativeDirect
Biological processEndocytosis0InformativeDirect
Biological processmRNA processing0.000002849InformativeDirect
Cellular componentMembrane0Least InformativeDirect
Cellular componentGolgi apparatus0Moderately InformativeDirect
Cellular componentEndosome0Moderately InformativeDirect
Cellular componentCytoplasmic vesicle0Moderately InformativeDirect
Cellular componentCoated pit0Highly InformativeDirect
DomainZinc-finger0.0000008092Moderately InformativeDirect
DomainCoiled coil0.00003662Moderately InformativeDirect
DomainSH3 domain0InformativeDirect
Molecular functionZinc0.0006226Least InformativeDirect
Molecular functionLipid-binding0Moderately InformativeDirect
Molecular functionActin-binding0.00000142InformativeDirect
Post-translational modificationPhosphoprotein0Least InformativeDirect
Post-translational modificationUbl conjugation0.00000000000001834Moderately InformativeDirect
Post-translational modificationIsopeptide bond0.00001026Moderately InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR008942 SSF48464 Protein matches
Abstract

This entry represents domains with a multi-helical, alpha-alpha 2-layered structural fold as found in: the ENTH domain of Epsin; the VHS domain of Hrs, Tom1, and ADP-ribosylation factors; the RPR domain of PCF11 protein; and the N-terminal domain of phosphoinositide-binding clathrin adaptor.

The epsin NH2-terminal homology (ENTH) domain is a membrane interacting module composed of a superhelix of alpha-helices. It is present at the NH2-terminus of proteins that often contain consensus sequences for binding to clathrin coat components and their accessory factors, and therefore function as endocytic adaptors. ENTH domain containing proteins have additional roles in signalling and actin regulation and may have yet other actions in the nucleus. The ENTH domain is structurally similar to the VHS domain.

The ENTH domain is approximately 150 amino acids long. The ENTH domain forms a compact globular structure, composed of eight alpha-helices connected by loops of varying length. Three helical hairpins that are stacked consecutively with a right-handed twist determine the general topology of the domain. This stacking gives the ENTH domain a rectangular appearance when viewed face on. The most highly conserved amino acids fall roughly into two classes: internal residues that are involved in packing and therefore are necessary for structural integrity, and solvent accessible residues that may be involved in protein-protein interactions [PubMed11911874].

VHS domains are found at the N-termini of select proteins involved in intracellular membrane trafficking. The domain consists of eight helices arranged in a superhelix. The surface of the domain has two main features: a basic patch on one side due to several conserved positively charged residues on helix 3 and a negatively charged ridge on the opposite side, formed by residues on helix 2. Comparison of the two VHS domains and the ENTH domain reveals a conserved surface, composed of helices 2 and 4, that is utilised for protein-protein interactions. In addition, VHS domain-containing proteins are also often localized to membranes. It has therefore been suggested that the conserved positively charged surface of helix 3 in VHS and ENTH domains plays a role in membrane binding [PubMed10985773].


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-coverage) · Mouse Phenotype (MP) · Worm Phenotype (WP) · Fly Anatomy (FA) · Zebrafish Anatomy (ZA) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 10 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a ENTH/VHS domain domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 10 hidden Markov models representing the ENTH/VHS domain superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-coverage) · Mouse Phenotype (MP) · Worm Phenotype (WP) · Fly Anatomy (FA) · Zebrafish Anatomy (ZA) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · UniProtKB KeyWords (KW) · Internal database links ]