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Subtilisin-like superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   Alpha and beta proteins (a/b) [ 51349] (147)
Fold:   Subtilisin-like [ 52742]
Superfamily:   Subtilisin-like [ 52743] (2)
Families:   Subtilases [ 52744] (13)
  Serine-carboxyl proteinase, SCP [ 52764]

Superfamily statistics
Genomes (2,149) Uniprot 2018_03 genome PDB chains (SCOP 1.75)
Domains 17,016 0 57
Proteins 16,794 0 57

Functional annotation
General category Processes_IC
Detailed category Proteases

Function annotation of SCOP domain superfamilies

Enzyme Commission (EC)

(show details)
EC termFDR (all)SDEO levelAnnotation (direct or inherited)
Enzyme Commission (EC)Acting on peptide bonds (peptide hydrolases)0Least InformativeDirect
Enzyme Commission (EC)Serine endopeptidases0Moderately InformativeDirect
Enzyme Commission (EC)Dipeptidyl-peptidases and tripeptidyl-peptidases0.00000000009456InformativeDirect
Enzyme Commission (EC)Furin0.00000000001063Highly InformativeDirect
Enzyme Commission (EC)Proprotein convertase 20.000000002004Highly InformativeDirect
Enzyme Commission (EC)Proprotein convertase 10.00000002702Highly InformativeDirect

Document: EC annotation of SCOP domains

Human Phenotype (HP)

(show details)
HP termFDR (all)SDHP levelAnnotation (direct or inherited)
Phenotypic Abnormality (PA)Abnormality of nervous system physiology0Least InformativeDirect
Phenotypic Abnormality (PA)Abnormality of nervous system morphology0Least InformativeDirect

Document: HP annotation of SCOP domains

Mouse Phenotype (MP)

(show details)
MP termFDR (all)SDMP levelAnnotation (direct or inherited)
Mammalian Phenotype (MP)mortality/aging0.1895Least InformativeInherited
Mammalian Phenotype (MP)prenatal lethality0.2016Moderately InformativeInherited
Mammalian Phenotype (MP)embryonic lethality prior to organogenesis0.0006973InformativeDirect

Document: MP annotation of SCOP domains

Worm Phenotype (WP)

(show details)
WP termFDR (all)SDWP levelAnnotation (direct or inherited)
Worm Phenotype (WP)larval development variant0Least InformativeDirect
Worm Phenotype (WP)larval growth variant0Least InformativeDirect
Worm Phenotype (WP)organ system morphology variant0.0006578Least InformativeDirect
Worm Phenotype (WP)pericellular component development variant0.0000001265Moderately InformativeDirect
Worm Phenotype (WP)organism segment morphology variant0.001918Moderately InformativeInherited
Worm Phenotype (WP)reproductive system morphology variant0.2588Moderately InformativeInherited
Worm Phenotype (WP)basement membrane remodeling variant0.00000004319InformativeDirect
Worm Phenotype (WP)body length variant0.0003718InformativeDirect
Worm Phenotype (WP)body width variant0.0004971InformativeDirect
Worm Phenotype (WP)dumpy0.00001758Highly InformativeDirect

Document: WP annotation of SCOP domains

Yeast Phenotype (YP)

(show details)
YP termFDR (all)SDYP levelAnnotation (direct or inherited)
Yeast Phenotype (YP)metabolism and growth0Least InformativeDirect
Yeast Phenotype (YP)resistance to chemicals0.7925Least InformativeInherited
Yeast Phenotype (YP)chemical compound accumulation0InformativeDirect
Yeast Phenotype (YP)acid pH resistance0.00008541Highly InformativeDirect

Document: YP annotation of SCOP domains

Fly Phenotype (FP)

(show details)
FP termFDR (all)SDFP levelAnnotation (direct or inherited)
Fly Phenotype (FP)increased mortality0Least InformativeDirect

Document: FP annotation of SCOP domains

Zebrafish Anatomy (ZA)

(show details)
ZA termFDR (all)SDZA levelAnnotation (direct or inherited)
Zebrafish Anatomy (ZA)nervous system0.3547Least InformativeInherited
Zebrafish Anatomy (ZA)multi-tissue structure0.3848Least InformativeInherited
Zebrafish Anatomy (ZA)compound organ0.5857Least InformativeInherited
Zebrafish Anatomy (ZA)central nervous system0.258Moderately InformativeInherited
Zebrafish Anatomy (ZA)brain0.1107InformativeInherited
Zebrafish Anatomy (ZA)optic tectum0.0006003Highly InformativeDirect

Document: ZA annotation of SCOP domains

Xenopus Anatomy (XA)

(show details)
XA termFDR (all)SDXA levelAnnotation (direct or inherited)
Xenopus ANatomical entity (XAN)cavitated compound organ0Least InformativeDirect
Xenopus ANatomical entity (XAN)embryo0.4594Least InformativeInherited
Xenopus ANatomical entity (XAN)vegetal hemisphere0.000014Highly InformativeDirect

Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details)
AP termFDR (all)SDAP levelAnnotation (direct or inherited)
Plant ANatomical entity (PAN)whole plant0.1509Least InformativeInherited
Plant ANatomical entity (PAN)collective phyllome structure0.6022Least InformativeInherited
Plant ANatomical entity (PAN)flower0.7989Least InformativeInherited
Plant ANatomical entity (PAN)cardinal part of multi-tissue plant structure0.9538Least InformativeInherited
Plant ANatomical entity (PAN)megasporophyll1Least InformativeInherited
Plant ANatomical entity (PAN)root system1Least InformativeInherited
Plant ANatomical entity (PAN)leaf1Least InformativeInherited
Plant ANatomical entity (PAN)style0.00001619Highly InformativeDirect
Plant ANatomical entity (PAN)phyllome vascular system0.00002069Highly InformativeDirect
Plant ANatomical entity (PAN)root stele0.00008333Highly InformativeDirect
Plant ANatomical entity (PAN)embryo sac cell0.0007186Highly InformativeDirect

Document: AP annotation of SCOP domains

Enzyme Commission (EC)

(show details)
EC termFDR (all)SDEC levelAnnotation (direct or inherited)
Enzyme Commission (EC)Hydrolases0Least InformativeDirect
Enzyme Commission (EC)Acting on peptide bonds (peptidases)0Moderately InformativeDirect
Enzyme Commission (EC)Serine endopeptidases0.00000000001295InformativeDirect
Enzyme Commission (EC)Dipeptidyl-peptidases and tripeptidyl-peptidases0Highly InformativeDirect

Document: EC annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Biological processVirulence0Moderately InformativeDirect
Biological processApoptosis0.0000000000199Moderately InformativeDirect
Biological processLipid metabolism0.0007384Moderately InformativeDirect
Biological processSporulation0InformativeDirect
Biological processSteroid metabolism0InformativeDirect
Biological processCholesterol metabolism0Highly InformativeDirect
Cellular componentGolgi apparatus0Moderately InformativeDirect
Cellular componentSecreted0Moderately InformativeDirect
Cellular componentEndosome0.0000000000003828Moderately InformativeDirect
Cellular componentCytoplasmic vesicle0.0000002363Moderately InformativeDirect
Cellular componentEndoplasmic reticulum0.000003092Moderately InformativeDirect
Cellular componentLysosome0InformativeDirect
DiseaseAllergen0.0002079Moderately InformativeDirect
DomainSignal0Least InformativeDirect
Molecular functionCalcium0Moderately InformativeDirect
Post-translational modificationHydrolase0Least InformativeDirect
Post-translational modificationProtease0Moderately InformativeDirect
Post-translational modificationSerine protease0InformativeDirect
Post-translational modificationGlycoprotein0Least InformativeDirect
Post-translational modificationCleavage on pair of basic residues0Moderately InformativeDirect
Post-translational modificationAutocatalytic cleavage0InformativeDirect
Post-translational modificationZymogen0InformativeDirect
Post-translational modificationSulfation0.00000000000001046InformativeDirect
Post-translational modificationPeptidoglycan-anchor0.00000000005602Highly InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000209 SSF52743 Protein matches

This group of serine peptidases belong to the MEROPS peptidase families S8 (subfamilies S8A (subtilisin) and S8B (kexin)) and S53 (sedolisin) both of which are members of clan SB.

The subtilisin family is the second largest serine protease family characterised to date. Over 200 subtilises are presently known, more than 170 of which with their complete amino acid sequence [PubMed9070434]. It is widespread, being found in eubacteria, archaebacteria, eukaryotes and viruses [PubMed7845208]. The vast majority of the family are endopeptidases, although there is an exopeptidase, tripeptidyl peptidase [PubMed7845208, PubMed8439290]. Structures have been determined for several members of the subtilisin family: they exploit the same catalytic triad as the chymotrypsins, although the residues occur in a different order (HDS in chymotrypsin and DHS in subtilisin), but the structures show no other similarity [PubMed7845208, PubMed8439290]. Some subtilisins are mosaic proteins, and others contain N- and C-terminal extensions that show no sequence similarity to any other known protein [PubMed7845208]. Based on sequence homology, a subdivision into six families has been proposed [PubMed9070434].

The proprotein-processing endopeptidases kexin, furin and related enzymes form a distinct subfamily known as the kexin subfamily (S8B). These preferentially cleave C-terminally to paired basic amino acids. Members of this subfamily can be identified by subtly different motifs around the active site [PubMed7845208, PubMed8439290]. Members of the kexin family, along with endopeptidases R, T and K from the yeast Tritirachium and cuticle-degrading peptidase from Metarhizium, require thiol activation. This can be attributed to the presence of Cys-173 near to the active histidine [PubMed8439290].Only 1 viral member of the subtilisin family is known, a 56-kDa protease from herpes virus 1, which infects the channel catfish [PubMed7845208].

Sedolisins (serine-carboxyl peptidases) are proteolytic enzymes whose fold resembles that of subtilisin; however, they are considerably larger, with the mature catalytic domains containing approximately 375 amino acids. The defining features of these enzymes are a unique catalytic triad, Ser-Glu-Asp, as well as the presence of an aspartic acid residue in the oxyanion hole. High-resolution crystal structures have now been solved for sedolisin from Pseudomonas sp. 101, as well as for kumamolisin from a thermophilic bacterium, Bacillus novo sp. MN-32. Mutations in the human gene leads to a fatal neurodegenerative disease [PubMed12673349].

InterPro database

PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Enzyme Commission (EC) · Human Phenotype (HP) · Mouse Phenotype (MP) · Worm Phenotype (WP) · Yeast Phenotype (YP) · Fly Phenotype (FP) · Zebrafish Anatomy (ZA) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · Enzyme Commission (EC) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.

Alignments of sequences to 17 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.

Browse and view proteins in genomes which have different domain combinations including a Subtilisin-like domain.

Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.

Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 17 hidden Markov models representing the Subtilisin-like superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Enzyme Commission (EC) · Human Phenotype (HP) · Mouse Phenotype (MP) · Worm Phenotype (WP) · Yeast Phenotype (YP) · Fly Phenotype (FP) · Zebrafish Anatomy (ZA) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · Enzyme Commission (EC) · UniProtKB KeyWords (KW) · Internal database links ]