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Subtilisin-like superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   Alpha and beta proteins (a/b) [ 51349] (147)
Fold:   Subtilisin-like [ 52742]
Superfamily:   Subtilisin-like [ 52743] (2)
Families:   Subtilases [ 52744] (13)
  Serine-carboxyl proteinase, SCP [ 52764]


Superfamily statistics
Genomes (2,149) Uniprot 2017_06 genome PDB chains (SCOP 1.75)
Domains 17,016 72,246 57
Proteins 16,794 71,676 57


Functional annotation
General category Processes_IC
Detailed category Proteases

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) multicellular organismal process 0.000238 Least Informative Direct
Biological Process (BP) macromolecule metabolic process 0 Least Informative Direct
Biological Process (BP) primary metabolic process 0.000000003188 Least Informative Direct
Biological Process (BP) organonitrogen compound metabolic process 0 Least Informative Direct
Biological Process (BP) biological regulation 0.2925 Least Informative Inherited
Biological Process (BP) cellular nitrogen compound metabolic process 1 Least Informative Inherited
Biological Process (BP) single-organism cellular process 1 Least Informative Inherited
Biological Process (BP) cellular component organization or biogenesis 1 Least Informative Inherited
Biological Process (BP) peptide metabolic process 0.0000004467 Moderately Informative Direct
Biological Process (BP) cellular catabolic process 0.000000004523 Moderately Informative Direct
Biological Process (BP) regulation of biological quality 0.00000161 Moderately Informative Direct
Biological Process (BP) tissue development 1 Moderately Informative Inherited
Biological Process (BP) animal organ development 1 Moderately Informative Inherited
Biological Process (BP) organic substance catabolic process 0.0146 Moderately Informative Inherited
Biological Process (BP) gene expression 0.005786 Moderately Informative Inherited
Biological Process (BP) organelle organization 1 Moderately Informative Inherited
Biological Process (BP) proteolysis 0 Informative Direct
Biological Process (BP) regulation of hormone levels 0.0000000001933 Informative Direct
Biological Process (BP) regulation of apoptotic process 0.00005045 Informative Direct
Biological Process (BP) cellular macromolecule catabolic process 0.0000000008126 Informative Direct
Biological Process (BP) embryo development 0.7317 Informative Inherited
Biological Process (BP) lysosome organization 0.00000001517 Highly Informative Direct
Biological Process (BP) regulation of neuron apoptotic process 0 Highly Informative Direct
Biological Process (BP) cellular protein catabolic process 0.00000000002449 Highly Informative Direct
Biological Process (BP) multicellular organismal homeostasis 0.00001646 Highly Informative Direct
Biological Process (BP) protein maturation 0 Highly Informative Direct
Biological Process (BP) anatomical structure homeostasis 0.0003789 Highly Informative Direct
Molecular Function (MF) hydrolase activity 0 Least Informative Direct
Molecular Function (MF) binding 1 Least Informative Inherited
Molecular Function (MF) amide binding 0.0001097 Informative Direct
Molecular Function (MF) macromolecular complex binding 0.00000000001936 Informative Direct
Molecular Function (MF) peptidase activity, acting on L-amino acid peptides 0.4278 Informative Inherited
Molecular Function (MF) serine hydrolase activity 0 Highly Informative Direct
Molecular Function (MF) peptide binding 0.00004417 Highly Informative Direct
Molecular Function (MF) exopeptidase activity 0.7866 Highly Informative Inherited
Cellular Component (CC) intracellular organelle part 0.9371 Least Informative Inherited
Cellular Component (CC) intracellular membrane-bounded organelle 0.08257 Least Informative Inherited
Cellular Component (CC) cytoplasmic part 0.002753 Least Informative Inherited
Cellular Component (CC) vacuole 0 Moderately Informative Direct
Cellular Component (CC) endomembrane system 0.000000000001072 Moderately Informative Direct
Cellular Component (CC) vesicle 0.000000002561 Moderately Informative Direct
Cellular Component (CC) intracellular organelle lumen 0.2177 Moderately Informative Inherited
Cellular Component (CC) lytic vacuole 0.00007324 Informative Direct
Cellular Component (CC) Golgi apparatus 0 Informative Direct
Cellular Component (CC) cell surface 0.000000000001041 Informative Direct
Cellular Component (CC) external encapsulating structure 0.000000002873 Informative Direct
Cellular Component (CC) endosome 0.005429 Informative Inherited
Cellular Component (CC) late endosome 0.0000000002902 Highly Informative Direct
Cellular Component (CC) plant-type cell wall 0.00007547 Highly Informative Direct

Document: GO annotation of SCOP domains

Enzyme Commission (EC)

(show details)
EC termFDR (all)SDEO levelAnnotation (direct or inherited)
Enzyme Commission (EC)Acting on peptide bonds (peptide hydrolases)0Least InformativeDirect
Enzyme Commission (EC)Serine endopeptidases0Moderately InformativeDirect
Enzyme Commission (EC)Dipeptidyl-peptidases and tripeptidyl-peptidases0.00000000009456InformativeDirect
Enzyme Commission (EC)Furin0.00000000001063Highly InformativeDirect
Enzyme Commission (EC)Proprotein convertase 20.000000002004Highly InformativeDirect
Enzyme Commission (EC)Proprotein convertase 10.00000002702Highly InformativeDirect

Document: EC annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Biological processVirulence0Moderately InformativeDirect
Biological processApoptosis0.00000000002151Moderately InformativeDirect
Biological processLipid metabolism0.000715Moderately InformativeDirect
Biological processSporulation0InformativeDirect
Biological processSteroid metabolism0InformativeDirect
Biological processCholesterol metabolism0Highly InformativeDirect
Cellular componentGolgi apparatus0Moderately InformativeDirect
Cellular componentSecreted0Moderately InformativeDirect
Cellular componentEndosome0.0000000000001466Moderately InformativeDirect
Cellular componentCytoplasmic vesicle0.0000001833Moderately InformativeDirect
Cellular componentEndoplasmic reticulum0.000002033Moderately InformativeDirect
Cellular componentLysosome0InformativeDirect
DiseaseAllergen0.000217Moderately InformativeDirect
DomainSignal0Least InformativeDirect
Molecular functionCalcium0Moderately InformativeDirect
Post-translational modificationHydrolase0Least InformativeDirect
Post-translational modificationProtease0Moderately InformativeDirect
Post-translational modificationSerine protease0InformativeDirect
Post-translational modificationGlycoprotein0Least InformativeDirect
Post-translational modificationCleavage on pair of basic residues0Moderately InformativeDirect
Post-translational modificationAutocatalytic cleavage0InformativeDirect
Post-translational modificationZymogen0InformativeDirect
Post-translational modificationSulfation0.00000000000001128InformativeDirect
Post-translational modificationPeptidoglycan-anchor0.00000000005658Highly InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000209 SSF52743 Protein matches
Abstract

This group of serine peptidases belong to the MEROPS peptidase families S8 (subfamilies S8A (subtilisin) and S8B (kexin)) and S53 (sedolisin) both of which are members of clan SB.

The subtilisin family is the second largest serine protease family characterised to date. Over 200 subtilises are presently known, more than 170 of which with their complete amino acid sequence [PubMed9070434]. It is widespread, being found in eubacteria, archaebacteria, eukaryotes and viruses [PubMed7845208]. The vast majority of the family are endopeptidases, although there is an exopeptidase, tripeptidyl peptidase [PubMed7845208, PubMed8439290]. Structures have been determined for several members of the subtilisin family: they exploit the same catalytic triad as the chymotrypsins, although the residues occur in a different order (HDS in chymotrypsin and DHS in subtilisin), but the structures show no other similarity [PubMed7845208, PubMed8439290]. Some subtilisins are mosaic proteins, and others contain N- and C-terminal extensions that show no sequence similarity to any other known protein [PubMed7845208]. Based on sequence homology, a subdivision into six families has been proposed [PubMed9070434].

The proprotein-processing endopeptidases kexin, furin and related enzymes form a distinct subfamily known as the kexin subfamily (S8B). These preferentially cleave C-terminally to paired basic amino acids. Members of this subfamily can be identified by subtly different motifs around the active site [PubMed7845208, PubMed8439290]. Members of the kexin family, along with endopeptidases R, T and K from the yeast Tritirachium and cuticle-degrading peptidase from Metarhizium, require thiol activation. This can be attributed to the presence of Cys-173 near to the active histidine [PubMed8439290].Only 1 viral member of the subtilisin family is known, a 56-kDa protease from herpes virus 1, which infects the channel catfish [PubMed7845208].

Sedolisins (serine-carboxyl peptidases) are proteolytic enzymes whose fold resembles that of subtilisin; however, they are considerably larger, with the mature catalytic domains containing approximately 375 amino acids. The defining features of these enzymes are a unique catalytic triad, Ser-Glu-Asp, as well as the presence of an aspartic acid residue in the oxyanion hole. High-resolution crystal structures have now been solved for sedolisin from Pseudomonas sp. 101, as well as for kumamolisin from a thermophilic bacterium, Bacillus novo sp. MN-32. Mutations in the human gene leads to a fatal neurodegenerative disease [PubMed12673349].


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-coverage) · Enzyme Commission (EC) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 17 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a Subtilisin-like domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 17 hidden Markov models representing the Subtilisin-like superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-coverage) · Enzyme Commission (EC) · UniProtKB KeyWords (KW) · Internal database links ]