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Thymidylate synthase/dCMP hydroxymethylase superfamily
SCOP classification
Superfamily statistics
Functional annotation
| General category | Metabolism |
| Detailed category | Nucleotide metabolism and transport |
Function annotation of SCOP domain superfamilies
InterPro annotation
| Cross references | IPR000398 SSF55831 Protein matches |
| Abstract | Thymidylate synthase [ 6996564, 2117882]
catalyzes the reductive methylation
of dUMP to dTMP with concomitant conversion of 5,10-methylenetetrahydrofolate
to dihydrofolate:
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
This provides the sole de novo pathway for
production of dTMP and is the only enzyme in folate metabolism in which the
5,10-methylenetetrahydrofolate is oxidised during one-carbon transfer [3099389].
The enzyme is essential for regulating the balanced supply of the 4 DNA
precursors in normal DNA replication: defects in the enzyme activity
affecting the regulation process cause various biological and genetic
abnormalities, such as thymineless death [2243092]. The enzyme is an important target for certain chemotherapeutic drugs.
Thymidylate synthase is an enzyme of about 30 to 35 Kd in most species except
in protozoan and plants where it exists as a bifunctional enzyme that includes
a dihydrofolate reductase domain [3099389].
A cysteine residue is involved in the catalytic mechanism (it covalently binds
the 5,6-dihydro-dUMP intermediate). The sequence around the active site of
this enzyme is conserved from phages to vertebrates. |
InterPro database
PDBeMotif information about ligands, sequence and structure motifs
PDBeMotif resource
Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation ]
Internal database links
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Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry
out SCOP domain assignments to all genomes at the superfamily level.
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Alignments of sequences to 10 models
in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical
are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.
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Browse and view proteins in genomes which have
different domain combinations including a Thymidylate synthase/dCMP hydroxymethylase domain.
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Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.
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Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.
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There are 10 hidden Markov models representing the Thymidylate synthase/dCMP hydroxymethylase superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.
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Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Internal database links ]
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