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DnaJ/Hsp40 cysteine-rich domain superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   Small proteins [ 56992] (90)
Fold:   DnaJ/Hsp40 cysteine-rich domain [ 57937]
Superfamily:   DnaJ/Hsp40 cysteine-rich domain [ 57938]
Families:   DnaJ/Hsp40 cysteine-rich domain [ 57939] (2)


Superfamily statistics
Genomes (3,166) Uniprot 2017_06 genome PDB chains (SCOP 1.75)
Domains 7,403 30,873 2
Proteins 7,385 30,797 2


Functional annotation
General category Information
Detailed category Transcription

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) biological regulation 0.9499 Least Informative Inherited
Biological Process (BP) macromolecule localization 0.0005994 Moderately Informative Direct
Biological Process (BP) regulation of molecular function 0.0015 Moderately Informative Inherited
Biological Process (BP) cellular localization 0.001996 Moderately Informative Inherited
Biological Process (BP) regulation of localization 0.1854 Moderately Informative Inherited
Biological Process (BP) regulation of response to stimulus 0.2854 Moderately Informative Inherited
Biological Process (BP) regulation of protein metabolic process 0.09235 Moderately Informative Inherited
Biological Process (BP) negative regulation of cellular process 0.2181 Moderately Informative Inherited
Biological Process (BP) regulation of signaling 0.2186 Moderately Informative Inherited
Biological Process (BP) negative regulation of metabolic process 0.1646 Moderately Informative Inherited
Biological Process (BP) regulation of cell communication 0.2157 Moderately Informative Inherited
Biological Process (BP) positive regulation of cellular process 0.4936 Moderately Informative Inherited
Biological Process (BP) negative regulation of cell death 0.00008127 Informative Direct
Biological Process (BP) negative regulation of cellular protein metabolic process 0.0003699 Informative Direct
Biological Process (BP) regulation of protein modification process 0.01057 Informative Inherited
Biological Process (BP) negative regulation of molecular function 0.003109 Informative Inherited
Biological Process (BP) protein localization to organelle 0.02489 Informative Inherited
Biological Process (BP) regulation of protein localization 0.002774 Informative Inherited
Biological Process (BP) regulation of transferase activity 0.01044 Informative Inherited
Biological Process (BP) regulation of peptide transport 0.001004 Informative Inherited
Biological Process (BP) negative regulation of response to stimulus 0.002176 Informative Inherited
Biological Process (BP) negative regulation of cell communication 0.002679 Informative Inherited
Biological Process (BP) regulation of intracellular signal transduction 0.012 Informative Inherited
Biological Process (BP) regulation of phosphorylation 0.004901 Informative Inherited
Biological Process (BP) regulation of apoptotic process 0.9369 Informative Inherited
Biological Process (BP) negative regulation of signaling 0.002671 Informative Inherited
Biological Process (BP) protein folding 0 Highly Informative Direct
Biological Process (BP) negative regulation of transferase activity 0.00000009036 Highly Informative Direct
Biological Process (BP) negative regulation of intracellular signal transduction 0.000009315 Highly Informative Direct
Biological Process (BP) negative regulation of protein modification process 0.000009806 Highly Informative Direct
Biological Process (BP) negative regulation of phosphate metabolic process 0.00001368 Highly Informative Direct
Biological Process (BP) regulation of MAP kinase activity 0.00001496 Highly Informative Direct
Biological Process (BP) positive regulation of apoptotic process 0.00002953 Highly Informative Direct
Biological Process (BP) regulation of cellular response to stress 0.00006383 Highly Informative Direct
Molecular Function (MF) binding 0.08419 Least Informative Inherited
Molecular Function (MF) molecular function regulator 0.000000000483 Moderately Informative Direct
Molecular Function (MF) enzyme activator activity 0.0001097 Informative Direct
Molecular Function (MF) chaperone binding 8.27e-16 Highly Informative Direct
Cellular Component (CC) intracellular membrane-bounded organelle 0.03805 Least Informative Inherited
Cellular Component (CC) cytoplasmic part 0.001517 Least Informative Inherited
Cellular Component (CC) membrane 0.1538 Least Informative Inherited
Cellular Component (CC) intracellular organelle part 0.9253 Least Informative Inherited
Cellular Component (CC) plastid 0.0000006974 Informative Direct
Cellular Component (CC) thylakoid 0.00000000004723 Highly Informative Direct

Document: GO annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Biological processDNA replication0InformativeDirect
Biological processStress response0InformativeDirect
Cellular componentCytoplasm0Least InformativeDirect
Cellular componentEndoplasmic reticulum1Moderately InformativeInherited
Cellular componentMicrosome0.0004524Highly InformativeDirect
DomainRepeat0Least InformativeDirect
DomainZinc-finger0Moderately InformativeDirect
Molecular functionMetal-binding0Least InformativeDirect
Molecular functionZinc0Least InformativeDirect
Post-translational modificationChaperone0InformativeDirect
Post-translational modificationMethylation0.00009223Moderately InformativeDirect
Post-translational modificationPrenylation0InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR001305 SSF57938 Protein matches
Abstract

Molecular chaperones are a diverse family of proteins that function to protect proteins in the intracellular milieu from irreversible aggregation during synthesis and in times of cellular stress. The bacterial molecular chaperone DnaK is an enzyme that couples cycles of ATP binding, hydrolysis, and ADP release by an N-terminal ATP-hydrolyzing domain to cycles of sequestration and release of unfolded proteins by a C-terminal substrate binding domain. Dimeric GrpE is the co-chaperone for DnaK, and acts as a nucleotide exchange factor, stimulating the rate of ADP release 5000-fold [PubMed8016869]. DnaK is itself a weak ATPase; ATP hydrolysis by DnaK is stimulated by its interaction with another co-chaperone, DnaJ. Thus the co-chaperones DnaJ and GrpE are capable of tightly regulating the nucleotide-bound and substrate-bound state of DnaK in ways that are necessary for the normal housekeeping functions and stress-related functions of the DnaK molecular chaperone cycle.

Besides stimulating the ATPase activity of DnaK through its J-domain, DnaJ also associates with unfolded polypeptide chains and prevents their aggregation [PubMed15063739]. Thus, DnaK and DnaJ may bind to one and the same polypeptide chain to form a ternary complex. The formation of a ternary complex may result in cis-interaction of the J-domain of DnaJ with the ATPase domain of DnaK. An unfolded polypeptide may enter the chaperone cycle by associating first either with ATP-liganded DnaK or with DnaJ. DnaK interacts with both the backbone and side chains of a peptide substrate; it thus shows binding polarity and admits only L-peptide segments. In contrast, DnaJ has been shown to bind both L- and D-peptides and is assumed to interact only with the side chains of the substrate.


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-coverage) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 2 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a DnaJ/Hsp40 cysteine-rich domain domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 2 hidden Markov models representing the DnaJ/Hsp40 cysteine-rich domain superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-coverage) · UniProtKB KeyWords (KW) · Internal database links ]