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Phosphofructokinase superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   Alpha and beta proteins (a/b) [ 51349] (147)
Fold:   Phosphofructokinase [ 53783]
Superfamily:   Phosphofructokinase [ 53784]
Families:   Phosphofructokinase [ 53785] (2)


Superfamily statistics
Genomes (2,369) Uniprot 2017_06 genome PDB chains (SCOP 1.75)
Domains 5,543 24,378 5
Proteins 4,714 21,905 5


Functional annotation
General category Metabolism
Detailed category Energy

Document:
Function annotation of SCOP domain superfamilies

Gene Ontology (high-quality)

(show details)
GO termFDR (singleton)FDR (all)SDFO levelAnnotation (direct or inherited)
Biological Process (BP)cellular aromatic compound metabolic process0.0000063360.00000000008334Least InformativeDirect
Biological Process (BP)heterocycle metabolic process0.0000066170.00000000005419Least InformativeDirect
Biological Process (BP)cellular nitrogen compound metabolic process0.000019820.00000000101Least InformativeDirect
Biological Process (BP)organic cyclic compound metabolic process0.000021550.0000000000002061Least InformativeDirect
Biological Process (BP)single-organism metabolic process0.000061590Least InformativeDirect
Biological Process (BP)single-organism cellular process0.00042730.00001062Least InformativeDirect
Biological Process (BP)primary metabolic process0.041320.1659Least InformativeInherited
Biological Process (BP)organonitrogen compound metabolic process0.0020810.0000001009Least InformativeInherited
Biological Process (BP)monocarboxylic acid metabolic process0.000000000000040190Moderately InformativeDirect
Biological Process (BP)generation of precursor metabolites and energy0.00000000000013970Moderately InformativeDirect
Biological Process (BP)purine-containing compound metabolic process0.0000000000010970Moderately InformativeDirect
Biological Process (BP)cofactor metabolic process0.0000000000079960Moderately InformativeDirect
Biological Process (BP)carbohydrate metabolic process0.00000000025870Moderately InformativeDirect
Biological Process (BP)carbohydrate derivative metabolic process0.00000000034040Moderately InformativeDirect
Biological Process (BP)single-organism catabolic process0.000000017690Moderately InformativeDirect
Biological Process (BP)organic substance catabolic process0.00000032750Moderately InformativeDirect
Biological Process (BP)nucleoside phosphate metabolic process0.0000068830Moderately InformativeDirect
Biological Process (BP)oxidoreduction coenzyme metabolic process00InformativeDirect
Biological Process (BP)ATP metabolic process00InformativeDirect
Biological Process (BP)pyridine-containing compound metabolic process00InformativeDirect
Biological Process (BP)carbohydrate catabolic process00InformativeDirect
Biological Process (BP)phosphorylation0.0001080.00000006302InformativeDirect
Biological Process (BP)glycolytic process0.0000011880Highly InformativeDirect
Molecular Function (MF)transferase activity0.00000000011970Least InformativeDirect
Molecular Function (MF)transferase activity, transferring phosphorus-containing groups00Moderately InformativeDirect
Molecular Function (MF)phosphotransferase activity, alcohol group as acceptor00InformativeDirect
Molecular Function (MF)kinase activity00InformativeDirect
Cellular Component (CC)cytoplasmic part0.0018560.00000007272Least InformativeInherited

Document: GO annotation of SCOP domains

Gene Ontology (high-coverage)

(show details)
GO term FDR (all) SDFO level Annotation (direct or inherited)
Biological Process (BP) cellular aromatic compound metabolic process 0.00000000008334 Least Informative Direct
Biological Process (BP) heterocycle metabolic process 0.00000000005419 Least Informative Direct
Biological Process (BP) cellular nitrogen compound metabolic process 0.00000000101 Least Informative Direct
Biological Process (BP) organic cyclic compound metabolic process 0.0000000000002061 Least Informative Direct
Biological Process (BP) single-organism metabolic process 0 Least Informative Direct
Biological Process (BP) single-organism cellular process 0.00001062 Least Informative Direct
Biological Process (BP) organonitrogen compound metabolic process 0.0000001009 Least Informative Direct
Biological Process (BP) primary metabolic process 0.1659 Least Informative Inherited
Biological Process (BP) response to stimulus 0.9167 Least Informative Inherited
Biological Process (BP) cellular component organization or biogenesis 0.7251 Least Informative Inherited
Biological Process (BP) monocarboxylic acid metabolic process 0 Moderately Informative Direct
Biological Process (BP) generation of precursor metabolites and energy 0 Moderately Informative Direct
Biological Process (BP) purine-containing compound metabolic process 0 Moderately Informative Direct
Biological Process (BP) cofactor metabolic process 0 Moderately Informative Direct
Biological Process (BP) carbohydrate metabolic process 0 Moderately Informative Direct
Biological Process (BP) carbohydrate derivative metabolic process 0 Moderately Informative Direct
Biological Process (BP) single-organism catabolic process 0 Moderately Informative Direct
Biological Process (BP) organic substance catabolic process 0 Moderately Informative Direct
Biological Process (BP) nucleoside phosphate metabolic process 0 Moderately Informative Direct
Biological Process (BP) protein complex biogenesis 0.00004258 Moderately Informative Direct
Biological Process (BP) macromolecular complex subunit organization 0.001034 Moderately Informative Inherited
Biological Process (BP) oxidation-reduction process 0.3685 Moderately Informative Inherited
Biological Process (BP) response to oxygen-containing compound 0.002827 Moderately Informative Inherited
Biological Process (BP) response to organic substance 0.003674 Moderately Informative Inherited
Biological Process (BP) cellular component assembly 0.01504 Moderately Informative Inherited
Biological Process (BP) oxidoreduction coenzyme metabolic process 0 Informative Direct
Biological Process (BP) ATP metabolic process 0 Informative Direct
Biological Process (BP) pyridine-containing compound metabolic process 0 Informative Direct
Biological Process (BP) carbohydrate catabolic process 0 Informative Direct
Biological Process (BP) phosphorylation 0.00000006302 Informative Direct
Biological Process (BP) monosaccharide metabolic process 0.002002 Informative Inherited
Biological Process (BP) glycolytic process 0 Highly Informative Direct
Biological Process (BP) glucose metabolic process 0.0000000000004103 Highly Informative Direct
Biological Process (BP) response to carbohydrate 0.0000000008735 Highly Informative Direct
Biological Process (BP) NAD metabolic process 1 Highly Informative Inherited
Biological Process (BP) monosaccharide catabolic process 0.0101 Highly Informative Inherited
Molecular Function (MF) transferase activity 0 Least Informative Direct
Molecular Function (MF) binding 1 Least Informative Inherited
Molecular Function (MF) transferase activity, transferring phosphorus-containing groups 0 Moderately Informative Direct
Molecular Function (MF) carbohydrate derivative binding 0.0000006943 Moderately Informative Direct
Molecular Function (MF) small molecule binding 0.000008958 Moderately Informative Direct
Molecular Function (MF) anion binding 0.008193 Moderately Informative Inherited
Molecular Function (MF) phosphotransferase activity, alcohol group as acceptor 0 Informative Direct
Molecular Function (MF) kinase activity 0 Informative Direct
Molecular Function (MF) purine ribonucleoside triphosphate binding 0.000000006995 Informative Direct
Molecular Function (MF) purine nucleotide binding 0.00000002658 Informative Direct
Molecular Function (MF) ribonucleotide binding 0.00000003327 Informative Direct
Molecular Function (MF) ATP binding 0.0000000002316 Highly Informative Direct
Cellular Component (CC) cytoplasmic part 0.00000007272 Least Informative Direct
Cellular Component (CC) transferase complex 0.0000000000004305 Moderately Informative Direct
Cellular Component (CC) cytosolic part 0.0000000000007684 Moderately Informative Direct
Cellular Component (CC) transferase complex, transferring phosphorus-containing groups 0 Informative Direct

Document: GO annotation of SCOP domains

Enzyme Commission (EC)

(show details)
EC termFDR (all)SDEO levelAnnotation (direct or inherited)
Enzyme Commission (EC)Phosphotransferases with an alcohol group as accep0Moderately InformativeDirect

Document: EC annotation of SCOP domains

UniProtKB KeyWords (KW)

(show details)
KW termFDR (all)SDKW levelAnnotation (direct or inherited)
Biological processGlycolysis0InformativeDirect
Cellular componentCytoplasm0Least InformativeDirect
Molecular functionMagnesium0Least InformativeDirect
Molecular functionMetal-binding0Least InformativeDirect
Molecular functionNucleotide-binding0Least InformativeDirect
Post-translational modificationTransferase0Least InformativeDirect
Post-translational modificationKinase0Moderately InformativeDirect
Post-translational modificationAcetylation0.0003771Least InformativeDirect

Document: KW annotation of SCOP domains

InterPro annotation
Cross references IPR000023 SSF53784 Protein matches
Abstract The enzyme-catalysed transfer of a phosphoryl group from ATP is an important reaction in a wide variety of biological processes [PubMed2953977]. One enzyme that utilises this reaction is phosphofructokinase (PFK), which catalyses the phosphorylation of fructose-6-phosphate to fructose-1,6- bisphosphate, a key regulatory step in the glycolytic pathway [PubMed12023862, PubMed7825568]. PFK exists as a homotetramer in bacteria and mammals (where each monomer possesses 2 similar domains), and as an octomer in yeast (where there are 4 alpha- (PFK1) and 4 beta-chains (PFK2), the latter, like the mammalian monomers, possessing 2 similar domains [PubMed7825568]).

PFK is ~300 amino acids in length, and structural studies of the bacterial enzyme have shown it comprises two similar (alpha/beta) lobes: one involved in ATP binding and the other housing both the substrate-binding site and the allosteric site (a regulatory binding site distinct from the active site, but that affects enzyme activity). The identical tetramer subunits adopt 2 different conformations: in a 'closed' state, the bound magnesium ion bridges the phosphoryl groups of the enzyme products (ADP and fructose-1,6- bisphosphate); and in an 'open' state, the magnesium ion binds only the ADP [PubMed2975709], as the 2 products are now further apart. These conformations are thought to be successive stages of a reaction pathway that requires subunit closure to bring the 2 molecules sufficiently close to react [PubMed2975709].

Deficiency in PFK leads to glycogenosis type VII (Tauri's disease), an autosomal recessive disorder characterised by severe nausea, vomiting, muscle cramps and myoglobinuria in response to bursts of intense or vigorous exercise [PubMed7825568]. Sufferers are usually able to lead a reasonably ordinary life by learning to adjust activity levels [PubMed7825568].


InterPro database


PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Enzyme Commission (EC) · UniProtKB KeyWords (KW) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.


Alignments of sequences to 3 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.


Browse and view proteins in genomes which have different domain combinations including a Phosphofructokinase domain.


Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.


Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 3 hidden Markov models representing the Phosphofructokinase superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.


Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Gene Ontology (high-quality) · Gene Ontology (high-coverage) · Enzyme Commission (EC) · UniProtKB KeyWords (KW) · Internal database links ]